TY - JOUR
T1 - Increased expression of one isoform of leucine-rich alpha-2-glycoprotein in peritoneal fluid of women with uterine leiomyomas
AU - Ferrero, Simone
AU - Gillott, David J.
AU - Remorgida, Valentino
AU - Anserini, Paola
AU - Ragni, Nicola
AU - Grudzinskas, Jurgis G.
PY - 2009/3
Y1 - 2009/3
N2 - Objective: To compare the peritoneal fluid (PF) proteome of women with and without uterine leiomyomas. Study design: PF samples were collected at laparoscopy from 14 women with uterine leiomyomas and 14 patients without leiomyomas who underwent tubal sterilization. PF samples were subjected to two-dimensional gel electrophoresis, silver stained, digitally captured, and compared by computerised analysis. Protein spots with aberrant expression in PF of women with leiomyomas were identified by liquid chromatography tandem mass spectrometry. Results: One isoform of leucine-rich alpha-2-glycoprotein (LRGm), one immunoglobulin light chain, and one unidentified protein (pI: 5.62; M r: 51.1 kDa) had significantly higher expression in PF of women with leiomyomas. Two isoforms of fibrinogen gamma chain had decreased expression in PF of women with uterine leiomyomas. Following Bonferroni correction for multiple comparisons, the aberrant expressions of LRGm and of one isoform of fibrinogen gamma chain was confirmed. The expression of these molecules was not affected by the phase of the menstrual cycle. Conclusions: Uterine leiomyomas are associated with changes in proteins present within the peritoneal fluid. The physiopathological relevance of the increased expression of LRGm in PF of women with uterine leiomyomas remains unclear.
AB - Objective: To compare the peritoneal fluid (PF) proteome of women with and without uterine leiomyomas. Study design: PF samples were collected at laparoscopy from 14 women with uterine leiomyomas and 14 patients without leiomyomas who underwent tubal sterilization. PF samples were subjected to two-dimensional gel electrophoresis, silver stained, digitally captured, and compared by computerised analysis. Protein spots with aberrant expression in PF of women with leiomyomas were identified by liquid chromatography tandem mass spectrometry. Results: One isoform of leucine-rich alpha-2-glycoprotein (LRGm), one immunoglobulin light chain, and one unidentified protein (pI: 5.62; M r: 51.1 kDa) had significantly higher expression in PF of women with leiomyomas. Two isoforms of fibrinogen gamma chain had decreased expression in PF of women with uterine leiomyomas. Following Bonferroni correction for multiple comparisons, the aberrant expressions of LRGm and of one isoform of fibrinogen gamma chain was confirmed. The expression of these molecules was not affected by the phase of the menstrual cycle. Conclusions: Uterine leiomyomas are associated with changes in proteins present within the peritoneal fluid. The physiopathological relevance of the increased expression of LRGm in PF of women with uterine leiomyomas remains unclear.
KW - Leiomyomas
KW - Leucine-rich alpha-2-glycoprotein
KW - Peritoneal fluid
KW - Proteomics
KW - Tandem mass spectrometry
KW - Two-dimensional gel electrophoresis
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U2 - 10.1007/s00404-008-0741-1
DO - 10.1007/s00404-008-0741-1
M3 - Article
C2 - 18665377
AN - SCOPUS:58849134183
VL - 279
SP - 365
EP - 371
JO - Archives of Gynecology
JF - Archives of Gynecology
SN - 0170-9925
IS - 3
ER -