Increased expression of the CD80 accessory molecule by alveolar macrophages in asthmatic subjects and its functional involvement in allergen presentation to autologous T(H2) lymphocytes

Samuele E. Burastero, Zulma Magnani, Claudio Confetti, Laura Abbruzzese, Susanna Oddera, Piero Balbo, Giovanni A. Rossi, Emanuele Crimi

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Alveolar macrophages (AMs) are more efficient antigen- presenting cells in allergic individuals than in nonatopic subjects. Objective: We studied whether this difference may be correlated to increased expression of membrane costimulatory molecules, such as the B7 molecules (CD80 and CD86). Methods: Eleven subjects with allergic asthma sensitized to Dermatophagoides pteronyssinus and 5 healthy nonatopic volunteers underwent bronchoalveolar lavage, and the costimulatory molecule expression on AMs was evaluated. Peripheral blood T cells, either freshly isolated or as established D pteronyssinus-specific cell lines, were cultured with autologous monocytes or AMs as antigen-presenting cells. In vitro allergen- induced proliferation and cytokine production were evaluated in the presence of B7-blocking reagents. Results: Allergic individuals had a significantly higher proportion of AMs expressing the CD80 molecule than control subjects (28.5% ± 14.8% vs 1.4% ± 1.2%; P <.001), whereas no difference was observed in CD86 expression (2.0% ± 2.3% vs 1.1% ± 0.6; P > .1). In a large proportion of the asthmatic subjects we studied, AMs were presenting soluble antigens (tetanus toxoid and streptolysin-O) to freshly isolated T cells more efficiently than AMs from nonatopic control subjects. Finally, both T-cell proliferation and cytokine production of D pteronyssinus-specific established T-cell lines were inhibited by a CD80-blocking antibody in a dose-dependent manner. Conclusion: Costimulation by means of CD80 expressed by AMs is probably involved in the amplification of the allergen-specific T-lymphocyte response in the airways of asthmatic subjects.

Original languageEnglish
Pages (from-to)1136-1142
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Volume103
Issue number6
DOIs
Publication statusPublished - 1999

Keywords

  • Allergy
  • Antigen presentation
  • Asthma
  • CD80
  • CD86
  • Costimulation
  • Monocyte-macrophages

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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