Increased expression of urokinase mRNA in bovine aortic endothelial cells treated with propranolol

Francesca Peracchia, Nadia Polentarutti, Francesco Colotta, Luciana Mussoni

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Propranolol, a β-adrenergic blocker agent widely used in a number of cardiovascular disorders, increases plasminogen activator (PA) activity in confluent bovine aortic endothelial cells (BAEC). This effect is time and dose dependent (10-100 μM). Hybridization studies with specific cDNA showed that propranolol was able to induce an increase in urokinase mRNA expression in a dose and time dependent manner. The propranolol effect seems to be specific for urokinase mRNA, because it does not affect a-actin mRNA expression. Cycloheximide, similarly to propranolol, also increases urokinase mRNA, indicating that the gene expression may be regulated by some rapidly turning over protein. When both compounds were used in combination, a superinduction phenomenon was observed.

Original languageEnglish
Pages (from-to)977-981
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume160
Issue number3
DOIs
Publication statusPublished - May 15 1989

Fingerprint

Endothelial cells
Urokinase-Type Plasminogen Activator
Propranolol
Endothelial Cells
Messenger RNA
Adrenergic Antagonists
Plasminogen Activators
Cycloheximide
Gene expression
Adrenergic Agents
Actins
Complementary DNA
Gene Expression
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Increased expression of urokinase mRNA in bovine aortic endothelial cells treated with propranolol. / Peracchia, Francesca; Polentarutti, Nadia; Colotta, Francesco; Mussoni, Luciana.

In: Biochemical and Biophysical Research Communications, Vol. 160, No. 3, 15.05.1989, p. 977-981.

Research output: Contribution to journalArticle

@article{a8f8fd11dba34274b9df5a26522528b4,
title = "Increased expression of urokinase mRNA in bovine aortic endothelial cells treated with propranolol",
abstract = "Propranolol, a β-adrenergic blocker agent widely used in a number of cardiovascular disorders, increases plasminogen activator (PA) activity in confluent bovine aortic endothelial cells (BAEC). This effect is time and dose dependent (10-100 μM). Hybridization studies with specific cDNA showed that propranolol was able to induce an increase in urokinase mRNA expression in a dose and time dependent manner. The propranolol effect seems to be specific for urokinase mRNA, because it does not affect a-actin mRNA expression. Cycloheximide, similarly to propranolol, also increases urokinase mRNA, indicating that the gene expression may be regulated by some rapidly turning over protein. When both compounds were used in combination, a superinduction phenomenon was observed.",
author = "Francesca Peracchia and Nadia Polentarutti and Francesco Colotta and Luciana Mussoni",
year = "1989",
month = "5",
day = "15",
doi = "10.1016/S0006-291X(89)80096-8",
language = "English",
volume = "160",
pages = "977--981",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Increased expression of urokinase mRNA in bovine aortic endothelial cells treated with propranolol

AU - Peracchia, Francesca

AU - Polentarutti, Nadia

AU - Colotta, Francesco

AU - Mussoni, Luciana

PY - 1989/5/15

Y1 - 1989/5/15

N2 - Propranolol, a β-adrenergic blocker agent widely used in a number of cardiovascular disorders, increases plasminogen activator (PA) activity in confluent bovine aortic endothelial cells (BAEC). This effect is time and dose dependent (10-100 μM). Hybridization studies with specific cDNA showed that propranolol was able to induce an increase in urokinase mRNA expression in a dose and time dependent manner. The propranolol effect seems to be specific for urokinase mRNA, because it does not affect a-actin mRNA expression. Cycloheximide, similarly to propranolol, also increases urokinase mRNA, indicating that the gene expression may be regulated by some rapidly turning over protein. When both compounds were used in combination, a superinduction phenomenon was observed.

AB - Propranolol, a β-adrenergic blocker agent widely used in a number of cardiovascular disorders, increases plasminogen activator (PA) activity in confluent bovine aortic endothelial cells (BAEC). This effect is time and dose dependent (10-100 μM). Hybridization studies with specific cDNA showed that propranolol was able to induce an increase in urokinase mRNA expression in a dose and time dependent manner. The propranolol effect seems to be specific for urokinase mRNA, because it does not affect a-actin mRNA expression. Cycloheximide, similarly to propranolol, also increases urokinase mRNA, indicating that the gene expression may be regulated by some rapidly turning over protein. When both compounds were used in combination, a superinduction phenomenon was observed.

UR - http://www.scopus.com/inward/record.url?scp=0024349594&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024349594&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(89)80096-8

DO - 10.1016/S0006-291X(89)80096-8

M3 - Article

C2 - 2730649

AN - SCOPUS:0024349594

VL - 160

SP - 977

EP - 981

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -