Increased expression of urokinase mRNA in bovine aortic endothelial cells treated with propranolol

Francesca Peracchia; Nadia Polentarutti; Francesco Colotta; Luciana Mussoni

doi:10.1016/S0006-291X(89)80096-8

Increased expression of urokinase mRNA in bovine aortic endothelial cells treated with propranolol

Francesca Peracchia, Nadia Polentarutti, Francesco Colotta, Luciana Mussoni

Istituto Clinico Humanitas

Research output: Contribution to journal › Article › peer-review

Abstract

Propranolol, a β-adrenergic blocker agent widely used in a number of cardiovascular disorders, increases plasminogen activator (PA) activity in confluent bovine aortic endothelial cells (BAEC). This effect is time and dose dependent (10-100 μM). Hybridization studies with specific cDNA showed that propranolol was able to induce an increase in urokinase mRNA expression in a dose and time dependent manner. The propranolol effect seems to be specific for urokinase mRNA, because it does not affect a-actin mRNA expression. Cycloheximide, similarly to propranolol, also increases urokinase mRNA, indicating that the gene expression may be regulated by some rapidly turning over protein. When both compounds were used in combination, a superinduction phenomenon was observed.

Original language	English
Pages (from-to)	977-981
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	160
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(89)80096-8
Publication status	Published - May 15 1989

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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abstract = "Propranolol, a β-adrenergic blocker agent widely used in a number of cardiovascular disorders, increases plasminogen activator (PA) activity in confluent bovine aortic endothelial cells (BAEC). This effect is time and dose dependent (10-100 μM). Hybridization studies with specific cDNA showed that propranolol was able to induce an increase in urokinase mRNA expression in a dose and time dependent manner. The propranolol effect seems to be specific for urokinase mRNA, because it does not affect a-actin mRNA expression. Cycloheximide, similarly to propranolol, also increases urokinase mRNA, indicating that the gene expression may be regulated by some rapidly turning over protein. When both compounds were used in combination, a superinduction phenomenon was observed.",

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AU - Polentarutti, Nadia

AU - Colotta, Francesco

AU - Mussoni, Luciana

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