Increased free malondialdehyde concentrations in smokers normalise with a mixed fruit and vegetable juice concentrate: A pilot study

Fabrizia Bamonti, Cristina Novembrino, Silvia Ippolito, Enzo Soresi, Alberto Ciani, Silvia Lonati, Elisabetta Scurati-Manzoni, Giuliana Cighetti

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Cigarette smoking, a cardiovascular risk factor leading to oxygen free radical formation, is involved in the development of serious pathological conditions. On the other hand, a healthy diet and adequate supplementation can help prevent many diseases. The aim of our study was to evaluate in healthy light smokers the effects of supplementation with mixed fruit and vegetable juice powder concentrate on homocysteine metabolism and oxidative status. Methods: In this pilot study, 32 healthy volunteers, 16 light smokers and 16 non-smokers, on twice daily supplementation were monitored at time zero and after 30 days. Plasma homocysteine, and serum vitamin B 12 and folate concentrations were measured by immunoenzymatic assays; reactive oxygen species, total antioxidant capacity and thiol groups by spectrophotometric methods; and total and free malondialdehyde concentrations by gas chromatography-mass spectrometry with isotopic dilution. Results: Baseline free malondialdehyde concentrations were significantly higher in smokers than in non-smokers and normalised after 30-day supplementation. Baseline results for all the other parameters remained unchanged after supplementation, with no significant differences between smokers and non-smokers. Conclusion: This is the first study showing a significant decrease in free malondialdehyde levels in light smokers after 1-month phytonutrient supplementation.

Original languageEnglish
Pages (from-to)391-395
Number of pages5
JournalClinical Chemistry and Laboratory Medicine
Volume44
Issue number4
DOIs
Publication statusPublished - Apr 1 2006

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Vegetables
Fruits
Malondialdehyde
Homocysteine
Reactive Oxygen Species
Phytochemicals
Vitamin B 12
Nutrition
Folic Acid
Sulfhydryl Compounds
Metabolism
Tobacco Products
Gas chromatography
Powders
Gas Chromatography-Mass Spectrometry
Dilution
Free Radicals
Mass spectrometry
Assays
Healthy Volunteers

Keywords

  • Homocysteine
  • Malondialdehyde
  • Oxidative stress
  • Phytonutrients
  • Smoking

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Increased free malondialdehyde concentrations in smokers normalise with a mixed fruit and vegetable juice concentrate : A pilot study. / Bamonti, Fabrizia; Novembrino, Cristina; Ippolito, Silvia; Soresi, Enzo; Ciani, Alberto; Lonati, Silvia; Scurati-Manzoni, Elisabetta; Cighetti, Giuliana.

In: Clinical Chemistry and Laboratory Medicine, Vol. 44, No. 4, 01.04.2006, p. 391-395.

Research output: Contribution to journalArticle

Bamonti, Fabrizia ; Novembrino, Cristina ; Ippolito, Silvia ; Soresi, Enzo ; Ciani, Alberto ; Lonati, Silvia ; Scurati-Manzoni, Elisabetta ; Cighetti, Giuliana. / Increased free malondialdehyde concentrations in smokers normalise with a mixed fruit and vegetable juice concentrate : A pilot study. In: Clinical Chemistry and Laboratory Medicine. 2006 ; Vol. 44, No. 4. pp. 391-395.
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