Increased generation of reactive oxygen species in isolated rat fatty liver during postischemic reoxygenation

Bruno Nardo, Paolo Caraceni, Patrizia Pasini, Marco Domenicali, Fausto Catena, Giuseppe Cavallari, Bruno Santoni, Elisabetta Maiolini, Ignazio Grattagliano, Gianluigi Vendemiale, Franco Trevisani, Aldo Roda, Mauro Bernardi, Antonino Cavallari

Research output: Contribution to journalArticlepeer-review

Abstract

Background. Whether fatty infiltration of the liver influences the generation of reactive oxygen species (ROS) during reperfusion is unclear. Thus, this study aimed to compare the ROS formation that occurs during postanoxic reoxygenation in isolated normal and fatty livers. Methods. Isolated livers from fed Sprague-Dawley rats with normal or fatty livers induced by a choline-deficient diet were reperfused at 37°C for 60 min with an oxygenated medium containing 10 μM of lucigenin after 1 hr of warm ischemia. Superoxide anion generation was assessed by the chemiluminescence (CLS) signal emitted from the organ surface. The hepatic content of malondialdehyde (MDA) and glutathione was determined at the end of reperfusion. Tissue injury was evaluated by the liver histology and the alanine aminotransferase (ALT) release in the perfusate. Results. CLS started rapidly with reoxygenation and it diffused to the whole organ in both groups. However, CLS emission was significantly higher in fatty liver (after 10 min: 812.425±39.898 vs. 294.525±21.068 photons/cm2/sec; P

Original languageEnglish
Pages (from-to)1816-1820
Number of pages5
JournalTransplantation
Volume71
Issue number12
Publication statusPublished - Jun 27 2001

ASJC Scopus subject areas

  • Transplantation
  • Immunology

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