TY - JOUR
T1 - Increased generation of reactive oxygen species in isolated rat fatty liver during postischemic reoxygenation
AU - Nardo, Bruno
AU - Caraceni, Paolo
AU - Pasini, Patrizia
AU - Domenicali, Marco
AU - Catena, Fausto
AU - Cavallari, Giuseppe
AU - Santoni, Bruno
AU - Maiolini, Elisabetta
AU - Grattagliano, Ignazio
AU - Vendemiale, Gianluigi
AU - Trevisani, Franco
AU - Roda, Aldo
AU - Bernardi, Mauro
AU - Cavallari, Antonino
PY - 2001/6/27
Y1 - 2001/6/27
N2 - Background. Whether fatty infiltration of the liver influences the generation of reactive oxygen species (ROS) during reperfusion is unclear. Thus, this study aimed to compare the ROS formation that occurs during postanoxic reoxygenation in isolated normal and fatty livers. Methods. Isolated livers from fed Sprague-Dawley rats with normal or fatty livers induced by a choline-deficient diet were reperfused at 37°C for 60 min with an oxygenated medium containing 10 μM of lucigenin after 1 hr of warm ischemia. Superoxide anion generation was assessed by the chemiluminescence (CLS) signal emitted from the organ surface. The hepatic content of malondialdehyde (MDA) and glutathione was determined at the end of reperfusion. Tissue injury was evaluated by the liver histology and the alanine aminotransferase (ALT) release in the perfusate. Results. CLS started rapidly with reoxygenation and it diffused to the whole organ in both groups. However, CLS emission was significantly higher in fatty liver (after 10 min: 812.425±39.898 vs. 294.525±21.068 photons/cm2/sec; P
AB - Background. Whether fatty infiltration of the liver influences the generation of reactive oxygen species (ROS) during reperfusion is unclear. Thus, this study aimed to compare the ROS formation that occurs during postanoxic reoxygenation in isolated normal and fatty livers. Methods. Isolated livers from fed Sprague-Dawley rats with normal or fatty livers induced by a choline-deficient diet were reperfused at 37°C for 60 min with an oxygenated medium containing 10 μM of lucigenin after 1 hr of warm ischemia. Superoxide anion generation was assessed by the chemiluminescence (CLS) signal emitted from the organ surface. The hepatic content of malondialdehyde (MDA) and glutathione was determined at the end of reperfusion. Tissue injury was evaluated by the liver histology and the alanine aminotransferase (ALT) release in the perfusate. Results. CLS started rapidly with reoxygenation and it diffused to the whole organ in both groups. However, CLS emission was significantly higher in fatty liver (after 10 min: 812.425±39.898 vs. 294.525±21.068 photons/cm2/sec; P
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M3 - Article
C2 - 11455263
AN - SCOPUS:0035958220
VL - 71
SP - 1816
EP - 1820
JO - Transplantation
JF - Transplantation
SN - 0041-1337
IS - 12
ER -