Increased hippocampal DNA oxidation in serotonin transporter deficient mice

R. Mössner, R. Dringen, A. M. Persico, B. Janetzky, O. Okladnova, D. Albert, M. Götz, J. Benninghoff, A. Schmitt, M. Gerlach, P. Riederer, K. P. Lesch

Research output: Contribution to journalArticlepeer-review

Abstract

The serotonin transporter (5HTT) is the molecule responsible for the high-affinity reuptake of 5HT from the synaptic cleft. Mice lacking the 5HTT exhibit highly elevated extracellular concentrations of 5HT. We assessed whether the glutathione detoxification system is altered in 5HTT-deficient mice. While levels of reduced and oxidized glutathione were unchanged, glutathione metabolising enzymes showed a differential pattern of modulation. Glutathione peroxidase was reduced in frontal cortex, brainstem, and cerebellum of 5HTT-deficient mice, though not to a statistically significant extent, while a putative isoform of the detoxifying enzyme glutathione-S-transferase pi was decreased in a number of brain regions, especially in brainstem. At the level of the DNA, we found an increase of oxidative DNA adducts in the hippocampus of 5HTT-deficient mice. Given the importance of the hippocampus in learning and memory, this may be the most important neurochemical consequence of the absence of the 5HTT.

Original languageEnglish
Pages (from-to)557-565
Number of pages9
JournalJournal of Neural Transmission
Volume109
Issue number5-6
DOIs
Publication statusPublished - 2002

Keywords

  • DNA oxidation
  • Glutathione
  • Knockout
  • Mouse
  • Serotonin transporter

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Increased hippocampal DNA oxidation in serotonin transporter deficient mice'. Together they form a unique fingerprint.

Cite this