Increased IL-6 gene expression and production in patients with common variable immunodeficiency

F. Pandolfi, R. Paganelli, A. Oliva, I. Quinti, V. Polidori, E. Fanales-Belasio, E. Guerra, F. Aiuti

Research output: Contribution to journalArticle

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Abstract

We have studied IL-6 gene expression and production by in vitro stimulated peripheral blood mononuclear cells (PBMC) isolated from common variable immunodeficiency (CVI) patients. A strong hybridization signal for the IL-6 probe was observed in mRNA extracted from phytohaemagglutinin (PHA)- and PHA/phorbol myristate acetate (PMA)-stimulated PBMC from most of 12 CVI patients analysed. IL-6 production by PHA-stimulated PBMC from 28 CVI patients was evaluated in ELISA and found to be significantly (P <0.0001) higher than in normal controls. IL-6 production, however, did not correlate with the lymphocyte populations examined, nor with the absolute number of monocytes. We have also showed that IL-6 was able to increase IgM secretion by several Epstein-Barr virus (EBV)-transformed cell lines derived from both normal donors and CVI patients, but it failed to modify substantially the amounts of IgM and IgG produced in vitro by PBMC derived from CVI patients and activated with pokeweed mitogen (PWM) or anti-IgM. Our data indicate that IL-6 gene expression and production is increased in CVI, but CVI cells do not respond to IL-6 with increased production of immunoglobulin.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalClinical and Experimental Immunology
Volume92
Issue number2
Publication statusPublished - 1993

Fingerprint

Common Variable Immunodeficiency
Interleukin-6
Gene Expression
Blood Cells
Phytohemagglutinins
Immunoglobulin M
Pokeweed Mitogens
Transformed Cell Line
Tetradecanoylphorbol Acetate
Human Herpesvirus 4
Immunoglobulins
Monocytes
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Tissue Donors
Lymphocytes
Messenger RNA

Keywords

  • common variable immunodeficiency
  • IL-6
  • immunoglobulin defect
  • tumour necrosis factor

ASJC Scopus subject areas

  • Immunology

Cite this

Pandolfi, F., Paganelli, R., Oliva, A., Quinti, I., Polidori, V., Fanales-Belasio, E., ... Aiuti, F. (1993). Increased IL-6 gene expression and production in patients with common variable immunodeficiency. Clinical and Experimental Immunology, 92(2), 239-244.

Increased IL-6 gene expression and production in patients with common variable immunodeficiency. / Pandolfi, F.; Paganelli, R.; Oliva, A.; Quinti, I.; Polidori, V.; Fanales-Belasio, E.; Guerra, E.; Aiuti, F.

In: Clinical and Experimental Immunology, Vol. 92, No. 2, 1993, p. 239-244.

Research output: Contribution to journalArticle

Pandolfi, F, Paganelli, R, Oliva, A, Quinti, I, Polidori, V, Fanales-Belasio, E, Guerra, E & Aiuti, F 1993, 'Increased IL-6 gene expression and production in patients with common variable immunodeficiency', Clinical and Experimental Immunology, vol. 92, no. 2, pp. 239-244.
Pandolfi F, Paganelli R, Oliva A, Quinti I, Polidori V, Fanales-Belasio E et al. Increased IL-6 gene expression and production in patients with common variable immunodeficiency. Clinical and Experimental Immunology. 1993;92(2):239-244.
Pandolfi, F. ; Paganelli, R. ; Oliva, A. ; Quinti, I. ; Polidori, V. ; Fanales-Belasio, E. ; Guerra, E. ; Aiuti, F. / Increased IL-6 gene expression and production in patients with common variable immunodeficiency. In: Clinical and Experimental Immunology. 1993 ; Vol. 92, No. 2. pp. 239-244.
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