Increased immunostimulatory activity conferred to antigen-presenting cells by exposure to antigen extract from hepatocellular carcinoma after radiofrequency thermal ablation

Alessandro Zerbini, Massimo Pilli, Francesco Fagnoni, Guido Pelosi, Maria Grazia Pizzi, Simona Schivazappa, Diletta Laccabue, Cristina Cavallo, Claudia Schianchi, Carlo Ferrari, Gabriele Missale

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Radiofrequency thermal ablation represents an effective treatment for hepatocellular carcinoma (HCC) and it can also exert an "adjuvant" effect on spontaneous antitumor T-cell responses, as suggested by human and animal studies. The adjuvant effect is thought to depend on the huge amount of necrotic tumor antigen made available to the immune system by HCC thermal ablation. In addition, radiofrequency thermal ablation may result in the release of local stimuli responsible for activation and maturation of antigen-presenting cells (APCs). To test this hypothesis, we studied APC maturation and function in 19 patients undergoing thermal ablation for HCC. Patients' monocytes induced to differentiate with granulocyte macrophage colony-stimulating factor (GM-CSF), or GM-CSF plus IL-4, were cocultured in vitro with tumor debris generated by radiofrequency thermal ablation. Expression of costimulatory molecules, lymphnode homing chemokine receptor, antigen presentation, and cytokine secretion were enhanced by incubation with HCC treated tissue as compared with untreated HCC and nontumor liver tissue. Moreover, HCC-specific T-cell responses could be induced by monocytes activated with GM-CSF and incubated with thermally ablated HCC tissue. HCC thermal ablation can create an antigenic source along with stimuli appropriate for maturation of APCs to induce HCC-specific T-cell responses. These results contribute to explain at least in part the adjuvant effect of HCC thermal ablation and suggest a novel strategy to induce maturation of APCs and their loading with HCC antigens for active immunotherapy protocols aimed at reducing HCC recurrence after thermal ablation.

Original languageEnglish
Pages (from-to)271-282
Number of pages12
JournalJournal of Immunotherapy
Volume31
Issue number3
DOIs
Publication statusPublished - Apr 2008

Fingerprint

Antigen-Presenting Cells
Hepatocellular Carcinoma
Hot Temperature
Antigens
Granulocyte-Macrophage Colony-Stimulating Factor
T-Lymphocytes
Monocytes
Active Immunotherapy
Chemokine Receptors
Antigen Presentation
Neoplasm Antigens
Interleukin-4
Immune System
Cytokines
Recurrence

Keywords

  • DC
  • HCC
  • RFA

ASJC Scopus subject areas

  • Cancer Research
  • Pharmacology
  • Immunology

Cite this

Increased immunostimulatory activity conferred to antigen-presenting cells by exposure to antigen extract from hepatocellular carcinoma after radiofrequency thermal ablation. / Zerbini, Alessandro; Pilli, Massimo; Fagnoni, Francesco; Pelosi, Guido; Pizzi, Maria Grazia; Schivazappa, Simona; Laccabue, Diletta; Cavallo, Cristina; Schianchi, Claudia; Ferrari, Carlo; Missale, Gabriele.

In: Journal of Immunotherapy, Vol. 31, No. 3, 04.2008, p. 271-282.

Research output: Contribution to journalArticle

Zerbini, A, Pilli, M, Fagnoni, F, Pelosi, G, Pizzi, MG, Schivazappa, S, Laccabue, D, Cavallo, C, Schianchi, C, Ferrari, C & Missale, G 2008, 'Increased immunostimulatory activity conferred to antigen-presenting cells by exposure to antigen extract from hepatocellular carcinoma after radiofrequency thermal ablation', Journal of Immunotherapy, vol. 31, no. 3, pp. 271-282. https://doi.org/10.1097/CJI.0b013e318160ff1c
Zerbini, Alessandro ; Pilli, Massimo ; Fagnoni, Francesco ; Pelosi, Guido ; Pizzi, Maria Grazia ; Schivazappa, Simona ; Laccabue, Diletta ; Cavallo, Cristina ; Schianchi, Claudia ; Ferrari, Carlo ; Missale, Gabriele. / Increased immunostimulatory activity conferred to antigen-presenting cells by exposure to antigen extract from hepatocellular carcinoma after radiofrequency thermal ablation. In: Journal of Immunotherapy. 2008 ; Vol. 31, No. 3. pp. 271-282.
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