Increased in vitro tetanus-induced production of HIV type 1 following in vivo immunization of HIV type 1-infected individuals with tetanus toxoid

M. A. Ostrowski, S. K. Stanley, J. S. Justement, K. Gantt, D. Goletti, A. S. Fauci

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Abstract

We have previously demonstrated that immunization of HIV-1-infected individuals with the common recall antigen, tetanus, induced transient increases in plasma viremia as well as an increased ability to isolate virus from CD8+ T cell-depleted peripheral blood mononuclear cells (PBMCs) under minimally stimulated culture conditions (IL-2 plus IL-4) postimmunization. In this study, HIV-1-infected individuals were immunized with tetanus toxoid and PBMCs were examined at multiple time points following immunization. Tetanus- induced production of virus was defined as an increased ability to isolate HIV-1 from CD8+ T cell-depleted PBMCs in vitro in the presence of tetanus antigen as opposed to no antigen or control antigen alone. Following immunization, in vitro tetanus-induced production of HIV-1 was observed in 8 of 13 (62%) patients compared to 2 of 13 (15%) patients prior to immunization. In four of these patients, virus could also be isolated from CD8+ T cell-depleted PBMCs in the presence of tetanus without the addition of any exogenous IL-2. Furthermore, virus could be isolated from the unfractionated PBMCs of two patients when tetanus antigen alone was added to the culture in the absence of added PHA or PHA blasts. HIV-1 was isolated predominantly from CD4+ T cells with a CD45RO+, CD25+ phenotype and was associated with a trend to elevated levels in culture supernatants of IFN- γ, IL-6, TNF-α, and IL-4. These findings have important implications with regard to the role of ongoing antigen-specific immune responses in the induction of HIV-1 expression in vivo.

Original languageEnglish
Pages (from-to)473-480
Number of pages8
JournalAIDS Research and Human Retroviruses
Volume13
Issue number6
Publication statusPublished - 1997

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Tetanus Toxoid
Tetanus
HIV-1
Immunization
Blood Cells
Antigens
Viruses
T-Lymphocytes
Interleukin-4
Interleukin-2
Viremia
Histocompatibility Antigens Class II
In Vitro Techniques
Interleukin-6
Phenotype

ASJC Scopus subject areas

  • Immunology
  • Virology

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Increased in vitro tetanus-induced production of HIV type 1 following in vivo immunization of HIV type 1-infected individuals with tetanus toxoid. / Ostrowski, M. A.; Stanley, S. K.; Justement, J. S.; Gantt, K.; Goletti, D.; Fauci, A. S.

In: AIDS Research and Human Retroviruses, Vol. 13, No. 6, 1997, p. 473-480.

Research output: Contribution to journalArticle

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