TY - JOUR
T1 - Increased infiltration of macrophages in omental adipose tissue is associated with marked hepatic lesions in morbid human obesity
AU - Cancello, Raffaella
AU - Tordjman, Joan
AU - Poitou, Christine
AU - Guilhem, Gaël
AU - Bouillot, Jean Luc
AU - Hugol, Danielle
AU - Coussieu, Christiane
AU - Basdevant, Arnaud
AU - Hen, Avner Bar
AU - Bedossa, Pierre
AU - Guerre-Millo, Michèle
AU - Clément, Karine
PY - 2006
Y1 - 2006
N2 - In human obesity, white adipose tissue (WAT) is enriched in macrophages. How macrophage infiltration in WAT contributes to the complications of obesity is unknown. This study tested the hypothesis that recruitment of macrophages in omental WAT is associated with hepatic damage in obese patients. Paired biopsies of subcutaneous and omental WAT and a liver biopsy were collected during gastric surgery in 46 obese women and 9 obese men (BMI 47.9 ± 0.93 kg/m2). The number of HAM56+ macrophages in WAT was quantified microscopically, and correlations with clinical and biological parameters and histological liver pathology were investigated. There were twice as many macrophages in omental as in subcutaneous WAT (P <0.0001). After adjustment for age, omental WAT macrophage infiltration was correlated to fasting glucose and insulin, quantitative insulin sensitivity check index, triglycerides, aspartate aminotransferase (AST), and γ-glutamyltranspeptidase. We propose an easy equation to estimate the amount of macrophages in omental WAT. Increased macrophage accumulation specifically in omental WAT was associated with hepatic fibroinflammatory lesions (P = 0.01). The best predictive model for the severity of hepatic damage includes adiponectinemia, AST, and omental WAT macrophages. These data suggest that the presence of macrophages in omental WAT participates in the cellular mechanisms favoring hepatic fibroinflammatory lesions in obese patients.
AB - In human obesity, white adipose tissue (WAT) is enriched in macrophages. How macrophage infiltration in WAT contributes to the complications of obesity is unknown. This study tested the hypothesis that recruitment of macrophages in omental WAT is associated with hepatic damage in obese patients. Paired biopsies of subcutaneous and omental WAT and a liver biopsy were collected during gastric surgery in 46 obese women and 9 obese men (BMI 47.9 ± 0.93 kg/m2). The number of HAM56+ macrophages in WAT was quantified microscopically, and correlations with clinical and biological parameters and histological liver pathology were investigated. There were twice as many macrophages in omental as in subcutaneous WAT (P <0.0001). After adjustment for age, omental WAT macrophage infiltration was correlated to fasting glucose and insulin, quantitative insulin sensitivity check index, triglycerides, aspartate aminotransferase (AST), and γ-glutamyltranspeptidase. We propose an easy equation to estimate the amount of macrophages in omental WAT. Increased macrophage accumulation specifically in omental WAT was associated with hepatic fibroinflammatory lesions (P = 0.01). The best predictive model for the severity of hepatic damage includes adiponectinemia, AST, and omental WAT macrophages. These data suggest that the presence of macrophages in omental WAT participates in the cellular mechanisms favoring hepatic fibroinflammatory lesions in obese patients.
KW - γGT, γ-glutamyltranspeptidase
KW - AST, aspartate aminotransferase
KW - NAFLD, nonalcoholic fatty liver disease
KW - QUICKI, quantitative insulin sensitivity check index
KW - TBS-TC, Tris-buffered saline/Tween 20/casein 0.02 mol/l solution
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U2 - 10.2337/db06-0133
DO - 10.2337/db06-0133
M3 - Article
C2 - 16731817
AN - SCOPUS:33748314535
VL - 55
SP - 1554
EP - 1561
JO - Diabetes
JF - Diabetes
SN - 0012-1797
IS - 6
ER -