Increased interferon-gamma (IFN-γ) levels produced in vitro by alloactivated T lymphocytes in systemic sclerosis and Raynaud's phenomenon

Monica Molteni, S. Della Bella, B. Mascagni, S. Bazzi, C. Zulian, S. Compasso, M. Lessi, R. Scorza

Research output: Contribution to journalArticle

Abstract

The aim of the present study was to analyse the in vitro proliferation and cytokine production by alloantigen-stimulated peripheral blood mononuclear cells (PBMC) obtained from patients affected by systemic sclerosis (SSc) and patients with Raynaud's phenomenon (RP). In SSc patients the proliferation of PBMC stimulated in vitro with alloantigens was significantly increased compared with healthy subjects, while no differences were observed for RP patients. Lymphocytes from SSc patients also produced larger amounts of IFN-γ compared with healthy controls. However, patients with clinically active disease had lower IFN-γ levels than those found in clinically stable patients. Patients affected by RP showed significantly higher levels of IFN-γ than healthy subjects. Analysis at the clonal level of the lymphocyte subsets involved in alloantigen stimulation in one patient affected by active SSc, and one subject with RP confirmed the results obtained using PBMC. In particular, in the RP patient but not in the SSc patient, we observed a population of CD4+ T cells which proliferated to alloantigens in vitro and produced high levels of IFN-γ. We suggest that T lymphocytes producing high levels of IFN-γ might play a protective role in RP patients and in established scleroderma.

Original languageEnglish
Pages (from-to)164-168
Number of pages5
JournalClinical and Experimental Immunology
Volume116
Issue number1
DOIs
Publication statusPublished - 1999

Keywords

  • Interferon-gamma
  • Raynaud's phenomenon
  • Systemic sclerosis
  • T lymphocytes

ASJC Scopus subject areas

  • Immunology

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