TY - JOUR
T1 - Increased Intestinal Permeability in Older Subjects Impacts the Beneficial Effects of Dietary Polyphenols by Modulating Their Bioavailability
AU - Hidalgo-Liberona, Nicole
AU - González-Domínguez, Raúl
AU - Vegas, Esteban
AU - Riso, Patrizia
AU - Del Bo, Cristian
AU - Bernardi, Stefano
AU - Peron, Gregorio
AU - Guglielmetti, Simone
AU - Gargari, Giorgio
AU - Kroon, Paul Antony
AU - Cherubini, Antonio
AU - Andrés-Lacueva, Cristina
N1 - Funding Information:
This work was accomplished as a part of the MaPLE project (Gut and Blood Microbiomics for Studying the Effect of a Polyphenol-Rich Dietary Pattern on Intestinal Permeability in the Elderly) supported within the European Joint Programming Initiative “A Healthy Diet for a Healthy Life” (JPI HDHL, http://www.healthydietforhealthylife.eu /), granted by Mipaaft (Italy, D.M. 8245/7303/2016), MINECO (Spain, PCIN-2015-238), and BBSRC (U.K., BB/R012512/1). This work also received funding from the JPI-HDHL ERA-Net Cofund on INtesTInal MICrobiomics (ERA-HDHL INTIMIC, AC19/00096), CIBERFES funded by Instituto de Salud Carlos III and co-funded by the European Regional Development Fund ″A way to make Europe″, and the Generalitat de Catalunya’s Agency AGAUR (2017SGR1546). Additional funding was provided by the Biotechnology and Biological Sciences Research Council (UK) through an Institute Strategic Programme Grant (‘Food Innovation and Health’; Grant No. BB/R012512/1) and its constituent projects BBS/E/F/000PR10343 (Theme 1, Food Innovation) and BBS/E/F/000PR10346 (Theme 3, Digestion and Fermentation in the Lower GI Tract) to the Quadram Institute Bioscience. RGD thanks the “Juan de la Cierva” program from MINECO (FJCI-2015-26590) and CAL the ICREA Academia award 2018. The sponsors of this work have no role in the development of the study protocol, methods, data collection and analyses, and the preparation of the manuscript.
Publisher Copyright:
© 2020 American Chemical Society.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/11/4
Y1 - 2020/11/4
N2 - Polyphenols have great potential in regulating intestinal health and ameliorating pathological conditions related to increased intestinal permeability (IP). However, the efficacy of dietary interventions with these phytochemicals may significantly be influenced by interindividual variability factors affecting their bioavailability and consequent biological activity. In the present study, urine samples collected from older subjects undergoing a crossover intervention trial with polyphenol-rich foods were subjected to metabolomics analysis for investigating the impact of increased IP on the bioavailability of polyphenols. Interestingly, urinary levels of phase II and microbiota-derived metabolites were significantly different between subjects with healthier intestinal barrier integrity and those with increased IP disruption. Our results support that this IP-dependent impaired bioavailability of polyphenols could be attributed to disturbances in the gut microbial metabolism and phase II methylation processes. Furthermore, we also observed that microbiota-derived metabolites could be largely responsible for the biological activity elicited by dietary polyphenols against age-related disrupted IP.
AB - Polyphenols have great potential in regulating intestinal health and ameliorating pathological conditions related to increased intestinal permeability (IP). However, the efficacy of dietary interventions with these phytochemicals may significantly be influenced by interindividual variability factors affecting their bioavailability and consequent biological activity. In the present study, urine samples collected from older subjects undergoing a crossover intervention trial with polyphenol-rich foods were subjected to metabolomics analysis for investigating the impact of increased IP on the bioavailability of polyphenols. Interestingly, urinary levels of phase II and microbiota-derived metabolites were significantly different between subjects with healthier intestinal barrier integrity and those with increased IP disruption. Our results support that this IP-dependent impaired bioavailability of polyphenols could be attributed to disturbances in the gut microbial metabolism and phase II methylation processes. Furthermore, we also observed that microbiota-derived metabolites could be largely responsible for the biological activity elicited by dietary polyphenols against age-related disrupted IP.
KW - aging
KW - bioavailability
KW - intestinal permeability
KW - metabolomics
KW - microbiota
KW - polyphenols
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U2 - 10.1021/acs.jafc.0c04976
DO - 10.1021/acs.jafc.0c04976
M3 - Article
C2 - 33084335
AN - SCOPUS:85095669497
VL - 68
SP - 12476
EP - 12484
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 44
ER -