Increased maternal/fetal blood S100B levels following systemic endotoxin administration and periventricular white matter injury in preterm fetal sheep

Yves Garnier, Alessandro Frigiola, Giovanni Li Volti, Pasquale Florio, Rosanna Frulio, Richard Berger, Stephanie Alm, Monika U. Von Duering, Audrey B C Coumans, Fernando M. Reis, Felice Petraglia, Tom H M Hasaart, Raoul Abella, Hala Mufeed, Diego Gazzolo

Research output: Contribution to journalArticle

Abstract

Objective. Intrauterine infection is suggested to cause perinatal brain white matter injury. In the current study, we evaluated whether S100B, a brain damage marker, may be also assessed in maternal bloodstream after white matter injury induced by fetal intravenous application of lypopolisaccharide (LPS) endotoxin. Methods. Fourteen fetal sheeps were chronically catheterized at a mean gestational age of 107 days. Three days after surgery, fetuses (n = 7) received 500 ng of LPS or 2 mL 0.9% saline (n = 7) intravenously (IV). Lypopolisaccharide and placebo groups were monitored by continuous hemodynamic data recordings and at 6 predetermined time points (control value; 3, 6, 24, 48, and 72 hours after LPS/placebo administration) blood was drawn for laboratory parameters and S100B assessment. Brain damage was evaluated by light microscopy after Klüver-Barrera staining. Selected areas of the periventricular white matter were also examined by electron microscopy. Results. White matter injury was detected in all LPS-treated fetuses, whereas no abnormalities were seen in control animals or in LPS-treated mothers. Maternal and fetal S100B protein levels were significantly higher in the LPS group than in the control group at all monitoring time points (P

Original languageEnglish
Pages (from-to)758-766
Number of pages9
JournalReproductive Sciences
Volume16
Issue number8
DOIs
Publication statusPublished - Aug 2009

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Keywords

  • Endotoxin.
  • Fetal brain damage
  • LPS
  • Perinatal infection
  • Preterm delivery
  • White matter injury

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Garnier, Y., Frigiola, A., Li Volti, G., Florio, P., Frulio, R., Berger, R., Alm, S., Von Duering, M. U., Coumans, A. B. C., Reis, F. M., Petraglia, F., Hasaart, T. H. M., Abella, R., Mufeed, H., & Gazzolo, D. (2009). Increased maternal/fetal blood S100B levels following systemic endotoxin administration and periventricular white matter injury in preterm fetal sheep. Reproductive Sciences, 16(8), 758-766. https://doi.org/10.1177/1933719109335801