Increased migration of a human glioma cell line after in vitro CyberKnife irradiation

Alessandra Canazza, Chiara Calatozzolo, Luisa Fumagalli, Achille Bergantin, Francesco Ghielmetti, Laura Fariselli, Danilo Croci, Andrea Salmaggi, Emilio Ciusani

Research output: Contribution to journalArticle

Abstract

A human glioblastoma multiforme cell line (U87) and its derived-spheroids were irradiated either using a conventional irradiation (CIR) or a CK-like irradiation (IIR) in which the 8 Gy was delivered intermittently over a period of 40 min. The ability of glioma cells to migrate into a matrigel matrix was evaluated on days 1-8 from irradiation. Irradiation with CKdriven IIR significantly increased the invasion potential of U87 cells in a matrigel-based assay. In contrast to CIR, IIR was associated with increased levels of TGFβ at four days (real-time PCR), β1-integrin at 4-5 d (real-time PCR and protein gel blot) and no elevation in phosphorylated AKT at days 4 and 5 (protein gel blot). Our data suggests that glioma cell invasion as well as elevations of TGFβ and β1-integrin are associated with IIR and not CIR.

Original languageEnglish
Pages (from-to)629-633
Number of pages5
JournalCancer Biology and Therapy
Volume12
Issue number7
DOIs
Publication statusPublished - Oct 1 2011

Keywords

  • Clonogenic survival
  • CyberKnife
  • Glioma
  • Invasion
  • Radiation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Molecular Medicine
  • Pharmacology

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