Increased migration of a human glioma cell line after in vitro CyberKnife irradiation

Alessandra Canazza, Chiara Calatozzolo, Luisa Fumagalli, Achille Bergantin, Francesco Ghielmetti, Laura Fariselli, Danilo Croci, Andrea Salmaggi, Emilio Ciusani

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A human glioblastoma multiforme cell line (U87) and its derived-spheroids were irradiated either using a conventional irradiation (CIR) or a CK-like irradiation (IIR) in which the 8 Gy was delivered intermittently over a period of 40 min. The ability of glioma cells to migrate into a matrigel matrix was evaluated on days 1-8 from irradiation. Irradiation with CKdriven IIR significantly increased the invasion potential of U87 cells in a matrigel-based assay. In contrast to CIR, IIR was associated with increased levels of TGFβ at four days (real-time PCR), β1-integrin at 4-5 d (real-time PCR and protein gel blot) and no elevation in phosphorylated AKT at days 4 and 5 (protein gel blot). Our data suggests that glioma cell invasion as well as elevations of TGFβ and β1-integrin are associated with IIR and not CIR.

Original languageEnglish
Pages (from-to)629-633
Number of pages5
JournalCancer Biology and Therapy
Volume12
Issue number7
DOIs
Publication statusPublished - Oct 1 2011

Fingerprint

Glioma
Integrins
Cell Line
Real-Time Polymerase Chain Reaction
Gels
Glioblastoma
Proteins
In Vitro Techniques
matrigel

Keywords

  • Clonogenic survival
  • CyberKnife
  • Glioma
  • Invasion
  • Radiation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Molecular Medicine
  • Pharmacology

Cite this

Increased migration of a human glioma cell line after in vitro CyberKnife irradiation. / Canazza, Alessandra; Calatozzolo, Chiara; Fumagalli, Luisa; Bergantin, Achille; Ghielmetti, Francesco; Fariselli, Laura; Croci, Danilo; Salmaggi, Andrea; Ciusani, Emilio.

In: Cancer Biology and Therapy, Vol. 12, No. 7, 01.10.2011, p. 629-633.

Research output: Contribution to journalArticle

Canazza, Alessandra ; Calatozzolo, Chiara ; Fumagalli, Luisa ; Bergantin, Achille ; Ghielmetti, Francesco ; Fariselli, Laura ; Croci, Danilo ; Salmaggi, Andrea ; Ciusani, Emilio. / Increased migration of a human glioma cell line after in vitro CyberKnife irradiation. In: Cancer Biology and Therapy. 2011 ; Vol. 12, No. 7. pp. 629-633.
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