Increased oxidative stress in dimethylnitrosamine-induced liver fibrosis in the rat: Effect of N-acetylcysteine and interferon-α

Gianluigi Vendemiale, Ignazio Grattagliano, Maria Lucia Caruso, Gaetano Serviddio, Anna Maria Valentini, Michele Pirrelli, Emanuele Altomare

Research output: Contribution to journalArticle

Abstract

Oxidative stress may represent a common link between chronic liver damage and hepatic fibrosis. Antioxidants and interferon seem to protect against hepatic stellate cell (HSC) activation and liver fibrosis. This study evaluated (1) the effect of the profibrotic agent dimethylnitrosamine (DMN) on the hepatic oxidative balance in the rat; (2) the role played by the antioxidant agent N-acetylcysteine (NAC); and (3) the antifibrotic effects of two different types of interferon-α: recombinant alpha-2b (rIFN-α) and leukocyte alpha (LeIFN-α). Five groups of rats received: (1) saline; (2) DMN; (3) DMN + NAC; (4) DMN + rIFN-α; and (5) DMN + LeIFN-α. Oxidative balance was evaluated by hepatic glutathione, TBARs, protein carbonyl, and sulfhydryl determination. Fibrosis was determined by hepatic hydroxyproline content and fibronectin (FN) staining (immunohistochemistry). DMN rats showed a diffuse FN deposition, an impaired oxidative balance, and higher hepatic hydroxyproline levels compared to that of controls. NAC administration significantly reduced FN deposition, increased hepatic glutathione, and decreased TBARs and protein carbonyls. Administration of IFN-α exerted different effects according to the type used. Both IFNs decreased FN deposition; however, LeIFN-α significantly improved histology and oxidative parameters compared to those of untreated DMN and rats treated with rIFN-α. This study shows the role of free radicals in this model of hepatic fibrosis; the protective effect of NAC against liver fibrosis; and the antifibrotic effect exerted by IFN-α (particularly LeIFN-α) independent of its antiviral activity.

Original languageEnglish
Pages (from-to)130-139
Number of pages10
JournalToxicology and Applied Pharmacology
Volume175
Issue number2
DOIs
Publication statusPublished - Sep 1 2001

Fingerprint

Dimethylnitrosamine
Oxidative stress
Acetylcysteine
Liver Cirrhosis
Liver
Interferons
Rats
Oxidative Stress
Fibronectins
interferon alfa-2b
Thiobarbituric Acid Reactive Substances
Hydroxyproline
Fibrosis
Glutathione
Antioxidants
Histology
Hepatic Stellate Cells
Free Radicals
Antiviral Agents
Proteins

Keywords

  • Dimethylnitrosamine
  • Interferon
  • Liver fibrosis
  • N-acetylcysteine
  • Oxidative balance

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Increased oxidative stress in dimethylnitrosamine-induced liver fibrosis in the rat : Effect of N-acetylcysteine and interferon-α. / Vendemiale, Gianluigi; Grattagliano, Ignazio; Caruso, Maria Lucia; Serviddio, Gaetano; Valentini, Anna Maria; Pirrelli, Michele; Altomare, Emanuele.

In: Toxicology and Applied Pharmacology, Vol. 175, No. 2, 01.09.2001, p. 130-139.

Research output: Contribution to journalArticle

Vendemiale, Gianluigi ; Grattagliano, Ignazio ; Caruso, Maria Lucia ; Serviddio, Gaetano ; Valentini, Anna Maria ; Pirrelli, Michele ; Altomare, Emanuele. / Increased oxidative stress in dimethylnitrosamine-induced liver fibrosis in the rat : Effect of N-acetylcysteine and interferon-α. In: Toxicology and Applied Pharmacology. 2001 ; Vol. 175, No. 2. pp. 130-139.
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AB - Oxidative stress may represent a common link between chronic liver damage and hepatic fibrosis. Antioxidants and interferon seem to protect against hepatic stellate cell (HSC) activation and liver fibrosis. This study evaluated (1) the effect of the profibrotic agent dimethylnitrosamine (DMN) on the hepatic oxidative balance in the rat; (2) the role played by the antioxidant agent N-acetylcysteine (NAC); and (3) the antifibrotic effects of two different types of interferon-α: recombinant alpha-2b (rIFN-α) and leukocyte alpha (LeIFN-α). Five groups of rats received: (1) saline; (2) DMN; (3) DMN + NAC; (4) DMN + rIFN-α; and (5) DMN + LeIFN-α. Oxidative balance was evaluated by hepatic glutathione, TBARs, protein carbonyl, and sulfhydryl determination. Fibrosis was determined by hepatic hydroxyproline content and fibronectin (FN) staining (immunohistochemistry). DMN rats showed a diffuse FN deposition, an impaired oxidative balance, and higher hepatic hydroxyproline levels compared to that of controls. NAC administration significantly reduced FN deposition, increased hepatic glutathione, and decreased TBARs and protein carbonyls. Administration of IFN-α exerted different effects according to the type used. Both IFNs decreased FN deposition; however, LeIFN-α significantly improved histology and oxidative parameters compared to those of untreated DMN and rats treated with rIFN-α. This study shows the role of free radicals in this model of hepatic fibrosis; the protective effect of NAC against liver fibrosis; and the antifibrotic effect exerted by IFN-α (particularly LeIFN-α) independent of its antiviral activity.

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