Increased percentage of activated Ia+ T lymphocytes in peripheral blood of neonates following exchange blood transfusion

M. Romano, A. El Marsafy, G. L. Marseglia, D. Rigal, B. Salle, J. L. Touraine

Research output: Contribution to journalArticlepeer-review

Abstract

The expression of Ia-like antigens in peripheral blood T lymphocytes from newborns receiving postnatal total blood exchange was analyzed. A significantly increased percentage of Ia-positive T lymphocytes (Ia+ T cells) was observed 2 days after postnatal transfusion with total blood in comparison to data observed on Days 0, 5, and 15. Ia+ T cells were also significantly higher than in normal control newborns tested in the same period. When newborns received the blood exchange with irradiated total blood or with leukocyte-depleted blood, no increase in Ia+ T cells was observed and the percentage of these cells remained in the normal range (1-7%) on all the days tested (0, 2, 5, 15 days). For easy identification of the origin of Ia+ T cells, sex-incompatible blood was used for exchange, and a karyotype analysis was carried out for the detection of the Y chromosome on Ia+ T cells separated from peripheral blood on Day 2 and then cultured with interleukin 2 (IL-2) for 48 hr. It was thus established that Ia+ T cells were not of donor origin. Simultaneously with the expression of Ia-like antigens, host T cells also carried the interleukin-2 receptor (TAC). An allogeneic response, comparable to a host-versusgraft reaction, was probably responsible for the activation of T cells 2 days after total blood exchange in newborns.

Original languageEnglish
Pages (from-to)301-307
Number of pages7
JournalClinical Immunology and Immunopathology
Volume43
Issue number3
DOIs
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

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