Increased plasma levels of nerve growth factor in vernal keratoconjunctivitis and relationship to conjunctival mast cells

A. Lambiase, S. Bonini, S. Bonini, A. Micera, L. Magrini, L. Bracci-Laudiero, L. Aloe

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose. To evaluate the nerve growth factor (NGF) plasma concentration in patients with vernal keratoconjunctivitis and to correlate it with the histopathology and immunopathology of the disease. Methods. An immunoenzymatic assay was performed to measure NGF plasma levels in patients with vernal keratoconjunctivitis and in healthy matched controls. A competitive radioimmunoassay was used to detect eosinophil cationic protein (ECP) and total and specific immunoglobulin E (IgE) serum levels. Histologic evaluation was performed in tarsal and bulbar conjunctival biopsies. Results. Plasma levels of NGF were significantly higher (P <0.001) in patients with vernal keratoconjunctivitis (mean = 8224.47 ± 7802.53 pg/ml; median = 121 pg/ml) than in controls (mean = 51.68 ± 5.94 pg/ml; median = 42.5 pg/ml). Conjunctival tissue showed a significant increase of mast cells, eosinophils, and lymphocytes in vernal keratoconjunctivitis. A significant correlation was observed between plasma levels of NGF and the number of mast cells in the tarsal conjunctiva (Cc = 0.81; P <0.005) and bulbar conjunctiva (Cc = 0.77; P <0.01) of patients with vernal keratoconjunctivitis. No correlation was found between NGF plasma levels and total IgE serum levels in patients with vernal keratoconjunctivitis; NGF plasma levels were inversely related to the number of circulating eosinophils (Cc = -0.61; P <0.05) and to the increased serum levels of ECP (Cc = -0.71; P <0.02). Conclusions. These data represent the first reported evidence of increased NGF plasma levels in an allergic human disease and suggest a possible relationship between this neurotrophic polypeptide and inflammatory cells in vernal keratoconjunctivitis.

Original languageEnglish
Pages (from-to)2127-2132
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume36
Issue number10
Publication statusPublished - 1995

Keywords

  • allergic disease
  • eosinophils
  • mast cells
  • nerve growth factor
  • vernal keratoconjunctivitis

ASJC Scopus subject areas

  • Ophthalmology

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