Increased production of platelet thromboxane B2 in non-insulin-dependent diabetes. Relationship to vascular complications

Platelet thromboxane B₂ production was studied in forty-seven non-insulin-dependent diabetics by incubating platelets with increasing concentrations of arachidonic acid. In comparison with thirty-two healthy subjects, diabetics showed increased thromboxane B₂ production at 0.7 mmol/l (mean: 236 pmol/10⁸ platelets, SEM 201-277; v. 135, 105-174; P <0.05) and at 1.0 mmol/l (673, 613-739; v. 405, 377-486, P <0.01) but not at 0.5 mmol/l. Patients were subdivided according to the presence or absence of vascular complications. Patients without microangiopathy showed significantly greater thromboxane B₂ production than healthy subjects at all the arachidonic-acid concentrations (P <0.02 or less). Patients with microangiopathy had platelet thromboxane production similar to that observed in healthy subjects at all the arachidonic-acid concentrations (P > 0.30) but significantly lower than that of non-microangiopathic patients at 0.5 (P <0.01) and at 0.7 mmol/l arachidonic acid (P <0.05). These results indicate that non-insulin-dependent diabetics have increased production of platelet thromboxane B₂ only when they do not have microvascular complications