Increased Renal Versican Expression Is Associated with Progression of Chronic Kidney Disease

Michael Rudnicki, Paul Perco, Hannes Neuwirt, Susie Jane Noppert, Johannes Leierer, Judith Sunzenauer, Susanne Eder, Carlamaria Zoja, Kathrin Eller, Alexander R. Rosenkranz, Gerhard A. Müller, Bernd Mayer, Gert Mayer

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Novel prognostic markers for progression of kidney disease are needed to distinguish patients who might benefit from a more aggressive nephroprotective therapy. Expression of the proteoglycan versican was evaluated in renal transcriptomics profiles and in an independent set of 74 renal biopsies. Versican levels were correlated to histologic damage scores and to renal outcome, and versican expression and regulation was evaluated in vitro. In transcriptomics profiles of renal tissue versican was positively correlated with (i) histological parameters in kidney biopsies, (ii) progressive decline of renal function in proteinuric kidney diseases, and (iii) impaired renal function and histology scores in diabetic nephropathy. In an independent cohort of 74 biopsies of glomerular diseases renal RNA levels of versican isoforms V0 and V1, but not V2 and V3 correlated significantly with creatinine after a mean follow up time of 53 months. Versican isoforms V0 and V1 together with serum creatinine at time of biopsy and the degree of glomerulosclerosis predicted 20% and 24% of the variability of creatinine at follow up, which was significantly more than serum creatinine and histological parameters alone (16%). However, when patients with acute kidney failure at time of biopsy (n = 5) were excluded, the additive predictive value of versican V1 was only marginally higher (35%) than creatinine and glomerulosclerosis alone (34%). Versican isoforms V0 and V1 were primarily expressed in vitro in proximal tubule cells and in fibroblasts. The results in humans were confirmed in three rodent models of kidney disease, in which renal versican expression was significantly upregulated as compared to corresponding controls. These data show for the first time an association of renal versican isoform V0 and V1 expression with progressive renal disease.

Original languageEnglish
Article numbere44891
JournalPLoS One
Volume7
Issue number9
DOIs
Publication statusPublished - Sep 14 2012

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Versicans
kidney diseases
Chronic Renal Insufficiency
kidneys
creatinine
Kidney
biopsy
Biopsy
Creatinine
Protein Isoforms
renal function
Kidney Diseases
transcriptomics
diabetic nephropathy
proximal tubules
proteoglycans
renal failure
histology
fibroblasts
Histology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Rudnicki, M., Perco, P., Neuwirt, H., Noppert, S. J., Leierer, J., Sunzenauer, J., ... Mayer, G. (2012). Increased Renal Versican Expression Is Associated with Progression of Chronic Kidney Disease. PLoS One, 7(9), [e44891]. https://doi.org/10.1371/journal.pone.0044891

Increased Renal Versican Expression Is Associated with Progression of Chronic Kidney Disease. / Rudnicki, Michael; Perco, Paul; Neuwirt, Hannes; Noppert, Susie Jane; Leierer, Johannes; Sunzenauer, Judith; Eder, Susanne; Zoja, Carlamaria; Eller, Kathrin; Rosenkranz, Alexander R.; Müller, Gerhard A.; Mayer, Bernd; Mayer, Gert.

In: PLoS One, Vol. 7, No. 9, e44891, 14.09.2012.

Research output: Contribution to journalArticle

Rudnicki, M, Perco, P, Neuwirt, H, Noppert, SJ, Leierer, J, Sunzenauer, J, Eder, S, Zoja, C, Eller, K, Rosenkranz, AR, Müller, GA, Mayer, B & Mayer, G 2012, 'Increased Renal Versican Expression Is Associated with Progression of Chronic Kidney Disease', PLoS One, vol. 7, no. 9, e44891. https://doi.org/10.1371/journal.pone.0044891
Rudnicki M, Perco P, Neuwirt H, Noppert SJ, Leierer J, Sunzenauer J et al. Increased Renal Versican Expression Is Associated with Progression of Chronic Kidney Disease. PLoS One. 2012 Sep 14;7(9). e44891. https://doi.org/10.1371/journal.pone.0044891
Rudnicki, Michael ; Perco, Paul ; Neuwirt, Hannes ; Noppert, Susie Jane ; Leierer, Johannes ; Sunzenauer, Judith ; Eder, Susanne ; Zoja, Carlamaria ; Eller, Kathrin ; Rosenkranz, Alexander R. ; Müller, Gerhard A. ; Mayer, Bernd ; Mayer, Gert. / Increased Renal Versican Expression Is Associated with Progression of Chronic Kidney Disease. In: PLoS One. 2012 ; Vol. 7, No. 9.
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