Increased resistance of peptides to serum proteases by modification of their amino groups

Rossella Galati, Alessandra Verdina, Giuliana Falasca, Alberto Chersi

Research output: Contribution to journalArticle

Abstract

The ability of synthetic protein fragments to survive the degradative action of aminopeptidases and serum proteolytic enzymes can be remarkably enhanced by slight modifications at their N-terminal alpha-amino group. This can be achieved by addition of beta-alanine or amino acids of the D-configuration, amino acids which are seldom found in a living organism. These modifications do scarcely modify the chemical and physical properties of the peptides, and should be preferrred, especially for in vivo tests, to drastic alterations of peptides as produced by dinitrophenylation or dansylation of the amino groups.

Original languageEnglish
Pages (from-to)558-561
Number of pages4
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume58
Issue number7-8
Publication statusPublished - Jul 2003

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Keywords

  • Modifications
  • Peptides
  • Proteolytic Degradation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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