Increased resistance to oxidative DNA damage of trabecular meshwork cells by E. coli FPG gene transfection

M. Foresta, G. Frosina, S. C. Sacca, C. Cartiglia, M. Longobardi, A. Izzotti

Research output: Contribution to journalArticle

Abstract

Oxidative damage plays a pathogenic role in various chronic degenerative diseases. Oxidative damage targeting trabecular meshwork (TM) cells as a consequence of mitochondrial damage is a pathogenic mechanism for glaucoma, the most common cause of irreversible blindness worldwide. Consequences of oxidative damage are attenuated by endocellular activities involved in scavenging reactive oxidative species and DNA repair. Selected bacterial genes are highly efficient at protecting cells from oxidative DNA damage. This situation occurs for Escherichia coli formamidopyrimidine DNA glycosylase (FPG), a major DNA glycosylase that repairs oxidatively damaged DNA. Accordingly, this study was aimed at transfecting human TM cells (HTMC) with Fpg in order to increase their resistance to oxidative damage. This study demonstrates that it is feasible to increase resistance of HTMC to endogenous oxidative damage by gene transfection. These findings bear relevance for primary and secondary prevention of degenerative glaucomas and other degenerative diseases where oxidative damage plays a pathogenic role.

Original languageEnglish
Pages (from-to)751-758
Number of pages8
JournalFree Radical Research
Volume45
Issue number7
DOIs
Publication statusPublished - Jul 2011

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Keywords

  • cell transfection
  • DNA repair
  • gene therapy
  • glaucoma
  • Oxidative DNA damage

ASJC Scopus subject areas

  • Biochemistry

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