Increased serum concentrations of soluble HLA-class I antigens in hepatitis C virus related mixed cryoglobulinaemia

Sergio Migliaresi, Giuseppe Tirri, Ciro Manzo, Alessandro Bresciani, Lucia Ambrosone, Marcantonio Spera, Deborah Barbarulo, Vincenza Lombari, Giuseppe Pirozzi, Guglielmo Borgia, Maria Luisa Lombardi

Research output: Contribution to journalArticlepeer-review

Abstract

Objective - To investigate whether quantitative alterations of both β2-microglobulin (β2μ) associated HLA class I heavy chains (sHLA-I) and β2 μ free class I heavy chains (sHLA-FHC) in sera of patients with hepatitis C virus (HCV) infection occur and whether they distinguish patients with mixed cryoglobulinaemia (MC). Methods - 83 HCV infected patients were studied and divided into three groups: (A) without cryoglobulinaemia (n=21), (B) with polyclonal MC (n=20), (C) with monoclonal MG (n=42). Serum sHLA-I and sHLA-FHC were measured by double determinant radioimmunoassay using monoclonal antibodies: TP25.99 as catching antibody, and NAMB-1 and HC-10 as revealing antibodies. Western blot identified HLA-I isoforms. Results - The serum concentrations of sHLA-I and of sHLA-FHC in HCV infected patients versus controls were respectively 1.3(0.5) μg/ml (mean (SD)) versus 0.8 (0.3) (p

Original languageEnglish
Pages (from-to)20-25
Number of pages6
JournalAnnals of the Rheumatic Diseases
Volume59
Issue number1
DOIs
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

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