Increased spinal cord NGF levels in rats with cobalamin (vitamin B 12) deficiency

Giuseppe Scalabrino, Elena Mutti, Daniela Veber, Luigi Aloe, Massimiliano M. Corsi, Stefania Galbiati, Giovanni Tredici

Research output: Contribution to journalArticle

Abstract

We have recently demonstrated that the neuropathological morphological alterations caused by cobalamin (Cbl) deficiency in the rat central nervous system are related to the vitamin's inability to modulate the synthesis of some neurotoxic and neurotrophic agents in opposite directions. In the present study, we measured nerve growth factor (NGF) levels in the spinal cord (SC) and cerebrospinal fluid (CSF) of rats made Cbl-deficient (Cbl-D) by means of total gastrectomy (TG) or a Cbl-D diet. In both cases, Cbl deficiency increased SC and CSF NGF levels after the appearance of myelinolytic lesions in the SC white matter (SCWM) (i.e. after the second post-TG month), and these changes were normalised by Cbl treatment in the 4-month-totally-gastrectomised (TGX) rats. Intracerebroventricular (i.c.v.) anti-NGF-antibody treatment prevented the onset of the myelinolytic SCWM lesions in the 2-month-TGX rats (i.e. when SC and CSF NGF levels are still normal) and normalised the ultrastructure of the SCWM in the 4-month-TGX rats, which was however worsened by the i.c.v. administration of NGF. These findings demonstrate that: (i) Cbl deficiency increases SC and CSF NGF levels; and (ii) endogenous NGF seems to play a noxious role in the progression of rat Cbl-D central neuropathy.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalNeuroscience Letters
Volume396
Issue number2
DOIs
Publication statusPublished - Mar 27 2006

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Vitamin B 12 Deficiency
Nerve Growth Factor
Vitamin B 12
Spinal Cord
Cerebrospinal Fluid
Gastrectomy
Vitamins
Central Nervous System
Diet
Antibodies

Keywords

  • Cerebrospinal fluid
  • Cobalamin
  • Nerve growth factor
  • Spinal cord
  • Subacute combined degeneration

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Increased spinal cord NGF levels in rats with cobalamin (vitamin B 12) deficiency. / Scalabrino, Giuseppe; Mutti, Elena; Veber, Daniela; Aloe, Luigi; Corsi, Massimiliano M.; Galbiati, Stefania; Tredici, Giovanni.

In: Neuroscience Letters, Vol. 396, No. 2, 27.03.2006, p. 153-158.

Research output: Contribution to journalArticle

Scalabrino, G, Mutti, E, Veber, D, Aloe, L, Corsi, MM, Galbiati, S & Tredici, G 2006, 'Increased spinal cord NGF levels in rats with cobalamin (vitamin B 12) deficiency', Neuroscience Letters, vol. 396, no. 2, pp. 153-158. https://doi.org/10.1016/j.neulet.2005.11.029
Scalabrino, Giuseppe ; Mutti, Elena ; Veber, Daniela ; Aloe, Luigi ; Corsi, Massimiliano M. ; Galbiati, Stefania ; Tredici, Giovanni. / Increased spinal cord NGF levels in rats with cobalamin (vitamin B 12) deficiency. In: Neuroscience Letters. 2006 ; Vol. 396, No. 2. pp. 153-158.
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