Increased susceptibility to peroxidation of VLDL from non-insulin-dependent diabetic patients: A possible correlation with fatty acid composition

R. A. Rabini, M. Tesei, T. Galeazzi, N. Dousset, G. Ferretti, L. Mazzanti

Research output: Contribution to journalArticle

Abstract

Recent studies suggested that both oxidized very low density lipoproteins (VLDL) and oxidized high density lipoproteins (HDL) might play a role in the pathogenesis of atherosclerosis. The aim of the present work was to analyse the susceptibility to in vitro peroxidation of VLDL and HDL from apparently normolipidemic subjects affected by insulin-dependent diabetes mellitus (IDDM) and non-insulin-dependent diabetes mellitus (NIDDM) in good metabolic control and to examine the possible relations between oxidisability and lipoprotein fatty acid composition. VLDL and HDL were isolated from 13 IDDM patients, 12 NIDDM patients and 18 healthy subjects. The degree of lipoprotein oxidation was determined by the measurement of hydroperoxide levels and thiobarbituric acid-reactive substances (TBARS) before and after in vitro peroxidative stress with CuSO4. Fatty acid analysis was performed by gas chromatography. VLDL and HDL from NIDDM patients showed a decrease in the saturated fatty acid content with a concomitant increase in unsaturated fatty acids and higher basal peroxide levels compared with healthy subjects. Oxidisability of VLDL from NIDDM subjects was higher than in controls and was significantly related with the unsaturated fatty acid content. The present work suggests that alterations in the composition and functions of both VLDL and HDL able to produce more atherogenic lipoproteins are present in NIDDM.

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalMolecular and Cellular Biochemistry
Volume199
Issue number1-2
DOIs
Publication statusPublished - 1999

Fingerprint

VLDL Lipoproteins
HDL Lipoproteins
Medical problems
Type 2 Diabetes Mellitus
Fatty Acids
Lipoproteins
Chemical analysis
Unsaturated Fatty Acids
Healthy Volunteers
Thiobarbituric Acid Reactive Substances
Peroxides
Insulin
Type 1 Diabetes Mellitus
Gas Chromatography
Hydrogen Peroxide
Atherosclerosis
Gas chromatography
Oxidation
In Vitro Techniques

Keywords

  • Diabetes mellitus
  • Fatty acid
  • HDL
  • Peroxidation
  • VLDL

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Increased susceptibility to peroxidation of VLDL from non-insulin-dependent diabetic patients : A possible correlation with fatty acid composition. / Rabini, R. A.; Tesei, M.; Galeazzi, T.; Dousset, N.; Ferretti, G.; Mazzanti, L.

In: Molecular and Cellular Biochemistry, Vol. 199, No. 1-2, 1999, p. 63-67.

Research output: Contribution to journalArticle

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