Abstract
TGFβ can modulate neuroblastoma (NB) cell proliferation and differentiation in vitro. In this study we used a NB cell line (LAN-5) which has been shown to partially respond to TGFβ and to present high levels of TGFβ receptor type I and low levels of receptor type II (TβRII) on the cell surface. To evaluate the role of TβRII in mediating TGFβ effects, LAN-5 cells were transfected with an expression vector containing the human full- length TβRII cDNA or with the empty vector pcDNA3. Compared to control CLV3 cells (transfected with empty plasmid) and parental LAN-5 cells, isolated neomycin-resistant clones (CL1 and CL3) expressed higher levels of TβRII, had reduced cell growth rate in vitro, and were unable to form tumors in vivo. Furthermore, isolated clones modified their morphology, assuming a terminally differentiated neuronal phenotype. Immunocytochemical staining demonstrated a basal increased expression of neural-specific markers, such as axonal growth-associated protein (GAP43) and neurofilaments (NF200). TGFβ treatment further increased the synthesis of NF200 and GAP43 in the transfected clones as revealed by Western blot analysis. These data indicate that TβRII overexpression potentiates the TGFβ signal transduction pathway, reverting NB cell neoplastic phenotype with the reduction of proliferation rate and the induction of terminal maturation. (C) 2000 Academic Press.
| Original language | English |
|---|---|
| Pages (from-to) | 77-85 |
| Number of pages | 9 |
| Journal | Experimental Cell Research |
| Volume | 255 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 25 2000 |
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Keywords
- Neuroblastoma cells
- Neuronal markers
- TβRII
- TGFβ
ASJC Scopus subject areas
- Cell Biology
Cite this
Increased TGFβ type II receptor expression suppresses the malignant phenotype and induces differentiation of human neuroblastoma cells. / Turco, Alessandra; Scarpa, Susanna; Coppa, Anna; Baccheschi, Gianluca; Palumbo, Camilla; Leonetti, Carlo; Zupi, Gabriella; Colletta, Giulia.
In: Experimental Cell Research, Vol. 255, No. 1, 25.02.2000, p. 77-85.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Increased TGFβ type II receptor expression suppresses the malignant phenotype and induces differentiation of human neuroblastoma cells
AU - Turco, Alessandra
AU - Scarpa, Susanna
AU - Coppa, Anna
AU - Baccheschi, Gianluca
AU - Palumbo, Camilla
AU - Leonetti, Carlo
AU - Zupi, Gabriella
AU - Colletta, Giulia
PY - 2000/2/25
Y1 - 2000/2/25
N2 - TGFβ can modulate neuroblastoma (NB) cell proliferation and differentiation in vitro. In this study we used a NB cell line (LAN-5) which has been shown to partially respond to TGFβ and to present high levels of TGFβ receptor type I and low levels of receptor type II (TβRII) on the cell surface. To evaluate the role of TβRII in mediating TGFβ effects, LAN-5 cells were transfected with an expression vector containing the human full- length TβRII cDNA or with the empty vector pcDNA3. Compared to control CLV3 cells (transfected with empty plasmid) and parental LAN-5 cells, isolated neomycin-resistant clones (CL1 and CL3) expressed higher levels of TβRII, had reduced cell growth rate in vitro, and were unable to form tumors in vivo. Furthermore, isolated clones modified their morphology, assuming a terminally differentiated neuronal phenotype. Immunocytochemical staining demonstrated a basal increased expression of neural-specific markers, such as axonal growth-associated protein (GAP43) and neurofilaments (NF200). TGFβ treatment further increased the synthesis of NF200 and GAP43 in the transfected clones as revealed by Western blot analysis. These data indicate that TβRII overexpression potentiates the TGFβ signal transduction pathway, reverting NB cell neoplastic phenotype with the reduction of proliferation rate and the induction of terminal maturation. (C) 2000 Academic Press.
AB - TGFβ can modulate neuroblastoma (NB) cell proliferation and differentiation in vitro. In this study we used a NB cell line (LAN-5) which has been shown to partially respond to TGFβ and to present high levels of TGFβ receptor type I and low levels of receptor type II (TβRII) on the cell surface. To evaluate the role of TβRII in mediating TGFβ effects, LAN-5 cells were transfected with an expression vector containing the human full- length TβRII cDNA or with the empty vector pcDNA3. Compared to control CLV3 cells (transfected with empty plasmid) and parental LAN-5 cells, isolated neomycin-resistant clones (CL1 and CL3) expressed higher levels of TβRII, had reduced cell growth rate in vitro, and were unable to form tumors in vivo. Furthermore, isolated clones modified their morphology, assuming a terminally differentiated neuronal phenotype. Immunocytochemical staining demonstrated a basal increased expression of neural-specific markers, such as axonal growth-associated protein (GAP43) and neurofilaments (NF200). TGFβ treatment further increased the synthesis of NF200 and GAP43 in the transfected clones as revealed by Western blot analysis. These data indicate that TβRII overexpression potentiates the TGFβ signal transduction pathway, reverting NB cell neoplastic phenotype with the reduction of proliferation rate and the induction of terminal maturation. (C) 2000 Academic Press.
KW - Neuroblastoma cells
KW - Neuronal markers
KW - TβRII
KW - TGFβ
UR - http://www.scopus.com/inward/record.url?scp=0034711879&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034711879&partnerID=8YFLogxK
U2 - 10.1006/excr.1999.4750
DO - 10.1006/excr.1999.4750
M3 - Article
C2 - 10666336
AN - SCOPUS:0034711879
VL - 255
SP - 77
EP - 85
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 1
ER -