IND-6, a highly divergent IND-type metallo-β-lactamase from Chryseobacterium indologenes strain 597 isolated in Burkina Faso

Boukaré Zeba, Filomena De Luca, Alain Dubus, Michael Delmarcelle, Jacques Simporé, Odile G. Nacoulma, Gian Maria Rossolini, Jean Marie Frère, Jean Denis Docquier

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The genus Chryseobacterium and other genera belonging to the family Flavobacteriaceae include organisms that can behave as human pathogens and are known to cause different kinds of infections. Several species of Flavobacteriaceae, including Chryseobacterium indologenes, are naturally resistant to β-lactam antibiotics (including carbapenems), due to the production of a resident metallo-β-lactamase. Although C. indologenes presently constitutes a limited clinical threat, the incidence of infections caused by this organism is increasing in some settings, where isolates that exhibit multidrug resistance phenotypes (including resistance to aminoglycosides and quinolones) have been detected. Here, we report the identification and characterization of a new IND-type variant from a C. indologenes isolate from Burkina Faso that is resistant to β-lactams and aminoglycosides. The levels of sequence identity of the new variant to other IND-type metallo-β- lactamases range between 72 and 90% (for IND-4 and IND-5, respectively). The purified enzyme exhibited N-terminal heterogeneity and a posttranslational modification consisting of the presence of a pyroglutamate residue at the N terminus. IND-6 shows a broad substrate profile, with overall higher turnover rates than IND-5 and higher activities than IND-2 and IND-5 against ceftazidime and cefepime.

Original languageEnglish
Pages (from-to)4320-4326
Number of pages7
JournalAntimicrobial Agents and Chemotherapy
Volume53
Issue number10
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Flavobacteriaceae
Chryseobacterium
Burkina Faso
Lactams
Aminoglycosides
Pyrrolidonecarboxylic Acid
Carbapenems
Ceftazidime
Quinolones
Multiple Drug Resistance
Post Translational Protein Processing
Infection
Phenotype
Incidence
Enzymes

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)
  • Pharmacology

Cite this

Zeba, B., De Luca, F., Dubus, A., Delmarcelle, M., Simporé, J., Nacoulma, O. G., ... Docquier, J. D. (2009). IND-6, a highly divergent IND-type metallo-β-lactamase from Chryseobacterium indologenes strain 597 isolated in Burkina Faso. Antimicrobial Agents and Chemotherapy, 53(10), 4320-4326. https://doi.org/10.1128/AAC.01607-08

IND-6, a highly divergent IND-type metallo-β-lactamase from Chryseobacterium indologenes strain 597 isolated in Burkina Faso. / Zeba, Boukaré; De Luca, Filomena; Dubus, Alain; Delmarcelle, Michael; Simporé, Jacques; Nacoulma, Odile G.; Rossolini, Gian Maria; Frère, Jean Marie; Docquier, Jean Denis.

In: Antimicrobial Agents and Chemotherapy, Vol. 53, No. 10, 10.2009, p. 4320-4326.

Research output: Contribution to journalArticle

Zeba, B, De Luca, F, Dubus, A, Delmarcelle, M, Simporé, J, Nacoulma, OG, Rossolini, GM, Frère, JM & Docquier, JD 2009, 'IND-6, a highly divergent IND-type metallo-β-lactamase from Chryseobacterium indologenes strain 597 isolated in Burkina Faso', Antimicrobial Agents and Chemotherapy, vol. 53, no. 10, pp. 4320-4326. https://doi.org/10.1128/AAC.01607-08
Zeba, Boukaré ; De Luca, Filomena ; Dubus, Alain ; Delmarcelle, Michael ; Simporé, Jacques ; Nacoulma, Odile G. ; Rossolini, Gian Maria ; Frère, Jean Marie ; Docquier, Jean Denis. / IND-6, a highly divergent IND-type metallo-β-lactamase from Chryseobacterium indologenes strain 597 isolated in Burkina Faso. In: Antimicrobial Agents and Chemotherapy. 2009 ; Vol. 53, No. 10. pp. 4320-4326.
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