Indirect immunofluorescence on HEp-2000 substrate is a sensitive method for detecting anti-Ro/SSA antibodies in patients with lupus erythematosus and/or photosensitivity

Emanuele Cozzani, Massimo Drosera, Marina Siccardi, Giovanni Babbini, Aurora Parodi

Research output: Contribution to journalArticlepeer-review

Abstract

Objective. To evaluate the sensitivity of indirect immunofluorescence (HF) utilizing HEp-2000 cells to detect antinuclear and anti-Ro/SSA antibodies. Methods. We examined 5949 sera samples from 2316 patients with connective tissue diseases. All sera were tested by HF on HEp-2 cells as substrate and by counterimmunoelectrophoresis (CIE). The positive sera for anti-Ro/SSA antibodies by CIE were retested by HF using HEp-2000 cells. The sera that were anti-Ro/SSA-positive in CIE but negative on HEp-2000 cells were tested by ELISA for the 60 and 52 subunits of Ro/SSA antigen. Results. Our results confirm the high sensitivity (81%) of HEp-2000 cells in detecting anti Ro/SSA antibodies. Conclusion. Using the HEp-2000 substrate is a reliable, simple alternative method for detecting anti-Ro/SSA antibodies in a first-level screening test such as HF, especially in patients with lupus erythematosus and/or photosensitivity.

Original languageEnglish
Pages (from-to)1320-1322
Number of pages3
JournalJournal of Rheumatology
Volume35
Issue number7
Publication statusPublished - Jul 2008

Keywords

  • Anti-Ro/SSA antibodies
  • HEp-2000 cells
  • High sensitivity
  • Lupus erythematosus

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

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