Abstract
Objective. To evaluate the sensitivity of indirect immunofluorescence (HF) utilizing HEp-2000 cells to detect antinuclear and anti-Ro/SSA antibodies. Methods. We examined 5949 sera samples from 2316 patients with connective tissue diseases. All sera were tested by HF on HEp-2 cells as substrate and by counterimmunoelectrophoresis (CIE). The positive sera for anti-Ro/SSA antibodies by CIE were retested by HF using HEp-2000 cells. The sera that were anti-Ro/SSA-positive in CIE but negative on HEp-2000 cells were tested by ELISA for the 60 and 52 subunits of Ro/SSA antigen. Results. Our results confirm the high sensitivity (81%) of HEp-2000 cells in detecting anti Ro/SSA antibodies. Conclusion. Using the HEp-2000 substrate is a reliable, simple alternative method for detecting anti-Ro/SSA antibodies in a first-level screening test such as HF, especially in patients with lupus erythematosus and/or photosensitivity.
| Original language | English |
|---|---|
| Pages (from-to) | 1320-1322 |
| Number of pages | 3 |
| Journal | Journal of Rheumatology |
| Volume | 35 |
| Issue number | 7 |
| Publication status | Published - Jul 2008 |
Keywords
- Anti-Ro/SSA antibodies
- HEp-2000 cells
- High sensitivity
- Lupus erythematosus
ASJC Scopus subject areas
- Rheumatology
- Immunology