Indirect resolution of β-blocker agents by reversed-phase capillary electrochromatography

Zeineb Aturki, Francesca Vichi, Antonella Messina, Massimo Sinibaldi

Research output: Contribution to journalArticle


The indirect resolution of five β-adrenoreceptor blocking agents (propranolol, oxprenolol, pindolol, metoprolol, and atenolol) using precolumn derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate (FLEC), and capillary electrochromatography (CEC) is reported. Three octadecylsilanized (ODS) silica gel-based stationary phases, differing in particle diameter and carbon surface density for suitability of determination of the enantiomeric composition of these substances in drugs and biological fluids, were studied. Under optimum separation conditions, all the investigated compounds were baseline-resolved within acceptable analysis times (i.e., between 10-16 min). The resolution values ranged between 1.80 and 1.14, and the separation factors were in no case less than 1.07. The possible use of the developed CEC method for the determination of enantiomeric composition of β-adrenoreceptor blocking agents in drug substances was investigated. A study, in which the enantiomers of metoprolol were examined regarding specificity, reproducibility, limit of detection, and ruggedness, was developed in accordance with some analytical procedures for method validation.

Original languageEnglish
Pages (from-to)607-614
Number of pages8
Issue number4-5
Publication statusPublished - Feb 2004


  • β-Blocker agents
  • (+)-1-(9-Fluorenyl)ethyl chloroformate
  • Capillar electrochromatography
  • Indirect resolution
  • Reversed-phase columns

ASJC Scopus subject areas

  • Clinical Biochemistry

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