Indole-3-acetic acid improves Escherichia coli's defences to stress

C. Bianco, E. Imperlini, R. Calogero, B. Senatore, A. Amoresano, A. Carpentieri, P. Pucci, R. Defez

Research output: Contribution to journalArticlepeer-review

Abstract

Indole-3-acetic acid (IAA) is a ubiquitous molecule playing regulatory roles in many living organisms. To elucidate the physiological changes induced by IAA treatment, we used Escherichia coli K-12 as a model system. By microarray analysis we found that 16 genes showed an altered expression level in IAA-treated cells. One-third of these genes encode cell envelope components, or proteins involved in bacterial adaptation to unfavourable environmental conditions. We thus investigated the effect of IAA treatment on some of the structural components of the envelope that may be involved in cellular response to stresses. This showed that IAA-treated cells had increased the production of trehalose, lipopolysaccharide (LPS), exopolysaccharide (EPS) and biofilm. We demonstrated further that IAA triggers an increased tolerance to several stress conditions (heat and cold shock, UV-irradiation, osmotic and acid shock and oxidative stress) and different toxic compounds (antibiotics, detergents and dyes) and this correlates with higher levels of the heat shock protein DnaK. We suggest that IAA triggers an increased level of alert and protection against external adverse conditions by coordinately enhancing different cellular defence systems.

Original languageEnglish
Pages (from-to)373-382
Number of pages10
JournalArchives of Microbiology
Volume185
Issue number5
DOIs
Publication statusPublished - Jun 2006

Keywords

  • Biofilm
  • DnaK
  • EPS
  • LPS
  • Stress response
  • Trehalose

ASJC Scopus subject areas

  • Microbiology

Fingerprint Dive into the research topics of 'Indole-3-acetic acid improves Escherichia coli's defences to stress'. Together they form a unique fingerprint.

Cite this