Indoleamine 2,3 dioxygenase gene polymorphisms correlate with CD8+ Treg impairment in systemic sclerosis

Samuele Tardito, Simone Negrini, Giuseppina Conteduca, Francesca Ferrera, Alessia Parodi, Florinda Battaglia, Francesca Kalli, Daniela Fenoglio, Maurizio Cutolo, Gilberto Filaci

Research output: Contribution to journalArticle

Abstract

Systemic sclerosis (SSc) is characterized by tissue fibrosis, vasculopathy and autoimmunity. Indoleamine 2,3 dioxygenase (IDO) plays a pivotal role in immunological tolerance modulating regulatory T cell (Treg) generation and function. Single nucleotide polymorphisms (SNPs) of IDO gene could impact on Treg function and predispose to autoimmunity. Here, the existence of an association between specific IDO SNPs and SSc was analyzed. Five specific SNPs in IDO gene were searched in 31 SSc patients and 37 healthy controls by gene sequencing or restriction fragment length polymorphism. The function of both CD4+CD25+ and CD8+ Treg from SSc patients was analyzed by proliferation suppression assay. SNP rs7820268 was statistically more frequent in SSc patients than in controls. Notably, SSc patients bearing the T allelic variant of the rs7820268 SNP showed impaired CD8+ Treg function. Our unprecedented data show that a specific IDO gene SNP is associated with an autoimmune disease such as SSc.

Original languageEnglish
Pages (from-to)166-169
Number of pages4
JournalHuman Immunology
Volume74
Issue number2
DOIs
Publication statusPublished - Feb 2013

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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    Tardito, S., Negrini, S., Conteduca, G., Ferrera, F., Parodi, A., Battaglia, F., Kalli, F., Fenoglio, D., Cutolo, M., & Filaci, G. (2013). Indoleamine 2,3 dioxygenase gene polymorphisms correlate with CD8+ Treg impairment in systemic sclerosis. Human Immunology, 74(2), 166-169. https://doi.org/10.1016/j.humimm.2012.11.008