Abstract
Indomethacin has been used to lower proteinuria in human glomerular diseases with controversial results. The mechanism of indomethacin beneficial effects has not been established. A possible explanation is that indomethacin reduces proteinuria by inhibiting the synthesis of renal prostaglandins (PGs); however, appropriate studies to address this issue have never been done. The objectives of the present study were: a) to investigate whether indomethacin influences protein excretion in an experimental model of immunological-mediated glomerular disease; b) to establish if the possible favorable effect of indomethacin on proteinuria is related to a reduction in glomerular filtration rate (GFR); c) to establish the possible association between the antiproteinuric effect of indomethacin and its inhibitory effect on arachidonic acid (AA) metabolites of renal or extrarenal origin; and d) to further investigate the relationship between proteinuria and renal thromboxane (Tx) synthesis previously demonstrated in experimental models of nephrotoxic nephritis and adriamycin (ADR) nephrosis. To this purpose we used an experimental immune-complex disease, passive Heymann nephritis (PHN) which was induced in the rat by a single intravenous (i.v.) injection of heterologous serum directed against a bruch border component (gp 330 antigen). Indomethacin at a dose of 6 mg/kg intraperitoneally (i.p.) administered for four consecutive days to PHN animals during the period of heavy proteinuria, effectively reduced urinary protein excretion. The reduction in proteinuria does not appear to be a consequence of a reduction in GFR as documented by inulin clearance. Glomerular synthesis and urinary excretion of vasodilatory prostacyclin (PGI2) and PGE2 were decreased or unchanged in PHN animals in respect to control animals. At variance, isolated glomeruli from rats with PHN studied 16 days after anti-gp 330 serum injection, when animals were heavily proteinuric, generated significantly more TxB2, the stable breakdown product of TxA2, than normal glomeruli. Urinary excretion of TxB2 was also significantly higher in rats with PHN than in normal rats. Indomethacin reduced the urinary TxB2 excretion by 74%. The administration of a selective Tx-synthase inhibitor UK-38,485 at a dose fully inhibiting platelet TxA2 synthesis only partially (around 40%) inhibited urinary excretion of TxB2 and did not reduce urinary protein excretion. These results may provide the experimental basis for the clinical observation that indomethacin has an antiproteinuric effect in humans.
| Original language | English |
|---|---|
| Pages (from-to) | 1335-1343 |
| Number of pages | 9 |
| Journal | Kidney International |
| Volume | 31 |
| Issue number | 6 |
| Publication status | Published - 1987 |
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ASJC Scopus subject areas
- Nephrology
Cite this
Indomethacin reduces proteinuria in passive Heymann nephritis in rats. / Zoja, C.; Benigni, A.; Verroust, P.; Ronco, P.; Bertani, T.; Remuzzi, G.
In: Kidney International, Vol. 31, No. 6, 1987, p. 1335-1343.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Indomethacin reduces proteinuria in passive Heymann nephritis in rats
AU - Zoja, C.
AU - Benigni, A.
AU - Verroust, P.
AU - Ronco, P.
AU - Bertani, T.
AU - Remuzzi, G.
PY - 1987
Y1 - 1987
N2 - Indomethacin has been used to lower proteinuria in human glomerular diseases with controversial results. The mechanism of indomethacin beneficial effects has not been established. A possible explanation is that indomethacin reduces proteinuria by inhibiting the synthesis of renal prostaglandins (PGs); however, appropriate studies to address this issue have never been done. The objectives of the present study were: a) to investigate whether indomethacin influences protein excretion in an experimental model of immunological-mediated glomerular disease; b) to establish if the possible favorable effect of indomethacin on proteinuria is related to a reduction in glomerular filtration rate (GFR); c) to establish the possible association between the antiproteinuric effect of indomethacin and its inhibitory effect on arachidonic acid (AA) metabolites of renal or extrarenal origin; and d) to further investigate the relationship between proteinuria and renal thromboxane (Tx) synthesis previously demonstrated in experimental models of nephrotoxic nephritis and adriamycin (ADR) nephrosis. To this purpose we used an experimental immune-complex disease, passive Heymann nephritis (PHN) which was induced in the rat by a single intravenous (i.v.) injection of heterologous serum directed against a bruch border component (gp 330 antigen). Indomethacin at a dose of 6 mg/kg intraperitoneally (i.p.) administered for four consecutive days to PHN animals during the period of heavy proteinuria, effectively reduced urinary protein excretion. The reduction in proteinuria does not appear to be a consequence of a reduction in GFR as documented by inulin clearance. Glomerular synthesis and urinary excretion of vasodilatory prostacyclin (PGI2) and PGE2 were decreased or unchanged in PHN animals in respect to control animals. At variance, isolated glomeruli from rats with PHN studied 16 days after anti-gp 330 serum injection, when animals were heavily proteinuric, generated significantly more TxB2, the stable breakdown product of TxA2, than normal glomeruli. Urinary excretion of TxB2 was also significantly higher in rats with PHN than in normal rats. Indomethacin reduced the urinary TxB2 excretion by 74%. The administration of a selective Tx-synthase inhibitor UK-38,485 at a dose fully inhibiting platelet TxA2 synthesis only partially (around 40%) inhibited urinary excretion of TxB2 and did not reduce urinary protein excretion. These results may provide the experimental basis for the clinical observation that indomethacin has an antiproteinuric effect in humans.
AB - Indomethacin has been used to lower proteinuria in human glomerular diseases with controversial results. The mechanism of indomethacin beneficial effects has not been established. A possible explanation is that indomethacin reduces proteinuria by inhibiting the synthesis of renal prostaglandins (PGs); however, appropriate studies to address this issue have never been done. The objectives of the present study were: a) to investigate whether indomethacin influences protein excretion in an experimental model of immunological-mediated glomerular disease; b) to establish if the possible favorable effect of indomethacin on proteinuria is related to a reduction in glomerular filtration rate (GFR); c) to establish the possible association between the antiproteinuric effect of indomethacin and its inhibitory effect on arachidonic acid (AA) metabolites of renal or extrarenal origin; and d) to further investigate the relationship between proteinuria and renal thromboxane (Tx) synthesis previously demonstrated in experimental models of nephrotoxic nephritis and adriamycin (ADR) nephrosis. To this purpose we used an experimental immune-complex disease, passive Heymann nephritis (PHN) which was induced in the rat by a single intravenous (i.v.) injection of heterologous serum directed against a bruch border component (gp 330 antigen). Indomethacin at a dose of 6 mg/kg intraperitoneally (i.p.) administered for four consecutive days to PHN animals during the period of heavy proteinuria, effectively reduced urinary protein excretion. The reduction in proteinuria does not appear to be a consequence of a reduction in GFR as documented by inulin clearance. Glomerular synthesis and urinary excretion of vasodilatory prostacyclin (PGI2) and PGE2 were decreased or unchanged in PHN animals in respect to control animals. At variance, isolated glomeruli from rats with PHN studied 16 days after anti-gp 330 serum injection, when animals were heavily proteinuric, generated significantly more TxB2, the stable breakdown product of TxA2, than normal glomeruli. Urinary excretion of TxB2 was also significantly higher in rats with PHN than in normal rats. Indomethacin reduced the urinary TxB2 excretion by 74%. The administration of a selective Tx-synthase inhibitor UK-38,485 at a dose fully inhibiting platelet TxA2 synthesis only partially (around 40%) inhibited urinary excretion of TxB2 and did not reduce urinary protein excretion. These results may provide the experimental basis for the clinical observation that indomethacin has an antiproteinuric effect in humans.
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M3 - Article
C2 - 2956450
AN - SCOPUS:0023254881
VL - 31
SP - 1335
EP - 1343
JO - Kidney International
JF - Kidney International
SN - 0085-2538
IS - 6
ER -