Inducible IL-2 production by dendritic cells revealed by global gene expression analysis

Francesca Granucci, Caterina Vizzardelli, Norman Pavelka, Sonia Feau, Maria Persico, Ettore Virzi, Maria Rescigno, Giorgio Moro, Paola Ricciardi-Castagnoli

Research output: Contribution to journalArticle

Abstract

Dendritic cells (DCs) are strong activators of primary T cell responses. Their priming ability is acquired upon encounter with maturation stimuli. To identify the genes that are differentially expressed upon maturation induced by exposure to Gram-negative bacteria, a kinetic study of DC gene expression was done with microarrays representing 11,000 genes and ESTs (expressed sequence tags). Approximately 3000 differentially expressed transcripts were identified. We found that functional interleukin 2 (IL-2) mRNA, which gave rise to IL-2 production, was transiently upregulated at early time-points after bacterial encounter. In contrast, macrophages did not produce IL-2 upon bacterial stimulation. Thus, IL-2 is an additional key cytokine that confers unique T cell stimulatory capacity to DCs.

Original languageEnglish
Pages (from-to)882-888
Number of pages7
JournalNature Immunology
Volume2
Issue number9
DOIs
Publication statusPublished - 2001

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Dendritic Cells
Interleukin-2
Gene Expression
T-Lymphocytes
Expressed Sequence Tags
Gram-Negative Bacteria
Genes
Macrophages
Cytokines
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Granucci, F., Vizzardelli, C., Pavelka, N., Feau, S., Persico, M., Virzi, E., ... Ricciardi-Castagnoli, P. (2001). Inducible IL-2 production by dendritic cells revealed by global gene expression analysis. Nature Immunology, 2(9), 882-888. https://doi.org/10.1038/ni0901-882

Inducible IL-2 production by dendritic cells revealed by global gene expression analysis. / Granucci, Francesca; Vizzardelli, Caterina; Pavelka, Norman; Feau, Sonia; Persico, Maria; Virzi, Ettore; Rescigno, Maria; Moro, Giorgio; Ricciardi-Castagnoli, Paola.

In: Nature Immunology, Vol. 2, No. 9, 2001, p. 882-888.

Research output: Contribution to journalArticle

Granucci, F, Vizzardelli, C, Pavelka, N, Feau, S, Persico, M, Virzi, E, Rescigno, M, Moro, G & Ricciardi-Castagnoli, P 2001, 'Inducible IL-2 production by dendritic cells revealed by global gene expression analysis', Nature Immunology, vol. 2, no. 9, pp. 882-888. https://doi.org/10.1038/ni0901-882
Granucci F, Vizzardelli C, Pavelka N, Feau S, Persico M, Virzi E et al. Inducible IL-2 production by dendritic cells revealed by global gene expression analysis. Nature Immunology. 2001;2(9):882-888. https://doi.org/10.1038/ni0901-882
Granucci, Francesca ; Vizzardelli, Caterina ; Pavelka, Norman ; Feau, Sonia ; Persico, Maria ; Virzi, Ettore ; Rescigno, Maria ; Moro, Giorgio ; Ricciardi-Castagnoli, Paola. / Inducible IL-2 production by dendritic cells revealed by global gene expression analysis. In: Nature Immunology. 2001 ; Vol. 2, No. 9. pp. 882-888.
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