Inducible lectins with galectin properties and human IL1α epitopes opsonize yeast during the inflammatory response of the ascidian Ciona intestinalis

Nicolò Parrinello, Vincenzo Arizza, Matteo Cammarata, Francesca Tiziana Giaramita, Margherita Pergolizzi, Mirella Vazzana, Aiti Vizzini, Daniela Parrinello

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Studies on inducible ascidian lectins may shed light on the evolutionary emergence of cytokine functions. Here, we show that the levels of opsonins, with IL1α-epitopes, increase in Ciona intestinalis hemolymph as a response to an inflammatory stimulus and, in particular, to intratunic injection of lipopolysaccharide (LPS). The inflammatory agent promptly (within 4 h) enhances Ca2+-independent serum hemagglutinating and opsonizing activities, which are both inhibited by D-galactose and D-galactosides (α-lactose, N-acetyl-D-lactosamine, thio-digalactoside), suggesting that anti-rabbit erythrocyte lectins with galectin properties are involved as opsonins. Inducible galectin molecules contain interleukin-1α (IL1α) epitopes, and their activities are specifically inhibited by anti-human recombinant IL1α antibody. Analysis by SDS-polyacrylamide gel electrophoresis has revealed that the density of the bands of several serum proteins increases within 4 h after LPS injection, correlated with the enhanced serum activity. Moreover, Western blot patterns demonstrate that several serum proteins (59, 37, 30, 23, 15 kDa) cross-react with the antibody as early as 4 h post-injection. Although we have not been able to establish whether, in adition to galectins, various types of D-galactose-specific lectins are contained in the serum, we show, for the first time in invertebrates, that galectin molecules with opsonic properties can be enhanced in response to a non-specific inflammatory stimulus, and that their release can be further stimulated by LPS. Finally, we reveal that multiple galectins share human IL1α epitopes, probably because of steric configuration and the oligomerization process.

Original languageEnglish
Pages (from-to)379-390
Number of pages12
JournalCell & Tissue Research
Volume329
Issue number2
DOIs
Publication statusPublished - Aug 2007

Fingerprint

Ciona intestinalis
Galectins
Urochordata
Interleukin-1
Lectins
Yeast
Epitopes
Yeasts
Opsonin Proteins
Lipopolysaccharides
Galactose
Injections
Blood Proteins
Serum
Galactosides
Oligomerization
Molecules
Hemolymph
Antibodies
Invertebrates

Keywords

  • Ascidian, Ciona intestinalis (Tunicata)
  • Evolution
  • IL1α-like galectins
  • Inflammatory response
  • Lectins
  • Opsonins
  • Phagocytosis

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Anatomy

Cite this

Inducible lectins with galectin properties and human IL1α epitopes opsonize yeast during the inflammatory response of the ascidian Ciona intestinalis. / Parrinello, Nicolò; Arizza, Vincenzo; Cammarata, Matteo; Giaramita, Francesca Tiziana; Pergolizzi, Margherita; Vazzana, Mirella; Vizzini, Aiti; Parrinello, Daniela.

In: Cell & Tissue Research, Vol. 329, No. 2, 08.2007, p. 379-390.

Research output: Contribution to journalArticle

Parrinello, N, Arizza, V, Cammarata, M, Giaramita, FT, Pergolizzi, M, Vazzana, M, Vizzini, A & Parrinello, D 2007, 'Inducible lectins with galectin properties and human IL1α epitopes opsonize yeast during the inflammatory response of the ascidian Ciona intestinalis', Cell & Tissue Research, vol. 329, no. 2, pp. 379-390. https://doi.org/10.1007/s00441-007-0415-5
Parrinello, Nicolò ; Arizza, Vincenzo ; Cammarata, Matteo ; Giaramita, Francesca Tiziana ; Pergolizzi, Margherita ; Vazzana, Mirella ; Vizzini, Aiti ; Parrinello, Daniela. / Inducible lectins with galectin properties and human IL1α epitopes opsonize yeast during the inflammatory response of the ascidian Ciona intestinalis. In: Cell & Tissue Research. 2007 ; Vol. 329, No. 2. pp. 379-390.
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