Abstract
Oxidative stress plays an important role in the early stage of acute pancreatitis as well as the associated multiple organ injury. Here we compare the degree of pancreatitis caused by cerulein in mice lacking the inducible (or type 2) nitric oxide synthase (iNOS) and in the corresponding wild-type mice. Intraperitoneal injection of cerulein resulted in wild-type mice in a severe, acute pancreatitis, which was characterized by edema, neutrophil infiltration, tissue hemorrhage and cell necrosis as well as increases in the serum levels of amylase and/or lipase. The infiltration of the pancreatic tissue of these animals with neutrophils (measured as increase in myeloperoxidase activity) was associated with up-regulation/expression of the adhesion molecules ICAM-1 and P-selectin as well as signs of enhanced lipid peroxidation (e.g., increased tissue levels of malondialdehyde). Immunohistochemical examination demonstrated a marked increase in the staining (immunoreactivity) for nitrotyrosine and poly (ADP-ribose) synthetase (PARS) in the pancreas of cerulein-treated iNOS wild-type mice. In contrast, the degree of pancreatic inflammation and tissue injury (histological score), upregulation/ expression of P-selectin and ICAM-1, the staining for nitrotyrosine and PARS, and lipid peroxidation was markedly reduced in pancreatic tissue sections obtained from cerulein-treated iNOS-deficient mice. These findings support the view that iNOS plays an important, pro-inflammatory role in the acute pancreatitis caused by cerulein in mice.
| Original language | English |
|---|---|
| Pages (from-to) | 416-422 |
| Number of pages | 7 |
| Journal | Shock |
| Volume | 17 |
| Issue number | 5 |
| Publication status | Published - May 2002 |
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Keywords
- Free radicals
- Inflammation
- iNOS
- Peroxynitrite
- Poly (ADP-ribose) polymerase
ASJC Scopus subject areas
- Physiology
- Critical Care and Intensive Care Medicine
Cite this
Inducible nitric oxide synthase-deficient mice exhibit resistance to the acute pancreatitis induced by cerulein. / Cuzzocrea, Salvatore; Mazzon, Emanuela; Dugo, Laura; Serraino, Ivana; Centorrino, Tommaso; Ciccolo, Antonio; Van De Loo, F. A J; Britti, Domenico; Caputi, Achille P.; Thiemermann, Christoph.
In: Shock, Vol. 17, No. 5, 05.2002, p. 416-422.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inducible nitric oxide synthase-deficient mice exhibit resistance to the acute pancreatitis induced by cerulein
AU - Cuzzocrea, Salvatore
AU - Mazzon, Emanuela
AU - Dugo, Laura
AU - Serraino, Ivana
AU - Centorrino, Tommaso
AU - Ciccolo, Antonio
AU - Van De Loo, F. A J
AU - Britti, Domenico
AU - Caputi, Achille P.
AU - Thiemermann, Christoph
PY - 2002/5
Y1 - 2002/5
N2 - Oxidative stress plays an important role in the early stage of acute pancreatitis as well as the associated multiple organ injury. Here we compare the degree of pancreatitis caused by cerulein in mice lacking the inducible (or type 2) nitric oxide synthase (iNOS) and in the corresponding wild-type mice. Intraperitoneal injection of cerulein resulted in wild-type mice in a severe, acute pancreatitis, which was characterized by edema, neutrophil infiltration, tissue hemorrhage and cell necrosis as well as increases in the serum levels of amylase and/or lipase. The infiltration of the pancreatic tissue of these animals with neutrophils (measured as increase in myeloperoxidase activity) was associated with up-regulation/expression of the adhesion molecules ICAM-1 and P-selectin as well as signs of enhanced lipid peroxidation (e.g., increased tissue levels of malondialdehyde). Immunohistochemical examination demonstrated a marked increase in the staining (immunoreactivity) for nitrotyrosine and poly (ADP-ribose) synthetase (PARS) in the pancreas of cerulein-treated iNOS wild-type mice. In contrast, the degree of pancreatic inflammation and tissue injury (histological score), upregulation/ expression of P-selectin and ICAM-1, the staining for nitrotyrosine and PARS, and lipid peroxidation was markedly reduced in pancreatic tissue sections obtained from cerulein-treated iNOS-deficient mice. These findings support the view that iNOS plays an important, pro-inflammatory role in the acute pancreatitis caused by cerulein in mice.
AB - Oxidative stress plays an important role in the early stage of acute pancreatitis as well as the associated multiple organ injury. Here we compare the degree of pancreatitis caused by cerulein in mice lacking the inducible (or type 2) nitric oxide synthase (iNOS) and in the corresponding wild-type mice. Intraperitoneal injection of cerulein resulted in wild-type mice in a severe, acute pancreatitis, which was characterized by edema, neutrophil infiltration, tissue hemorrhage and cell necrosis as well as increases in the serum levels of amylase and/or lipase. The infiltration of the pancreatic tissue of these animals with neutrophils (measured as increase in myeloperoxidase activity) was associated with up-regulation/expression of the adhesion molecules ICAM-1 and P-selectin as well as signs of enhanced lipid peroxidation (e.g., increased tissue levels of malondialdehyde). Immunohistochemical examination demonstrated a marked increase in the staining (immunoreactivity) for nitrotyrosine and poly (ADP-ribose) synthetase (PARS) in the pancreas of cerulein-treated iNOS wild-type mice. In contrast, the degree of pancreatic inflammation and tissue injury (histological score), upregulation/ expression of P-selectin and ICAM-1, the staining for nitrotyrosine and PARS, and lipid peroxidation was markedly reduced in pancreatic tissue sections obtained from cerulein-treated iNOS-deficient mice. These findings support the view that iNOS plays an important, pro-inflammatory role in the acute pancreatitis caused by cerulein in mice.
KW - Free radicals
KW - Inflammation
KW - iNOS
KW - Peroxynitrite
KW - Poly (ADP-ribose) polymerase
UR - http://www.scopus.com/inward/record.url?scp=0036583591&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036583591&partnerID=8YFLogxK
M3 - Article
C2 - 12022764
AN - SCOPUS:0036583591
VL - 17
SP - 416
EP - 422
JO - Shock
JF - Shock
SN - 1073-2322
IS - 5
ER -