Induction and regulation of cytotoxic T cells by microbial antigens and recombinant interleukin 2.

E. Piccolella, G. Lombardi, M. Gobbi, M. S. Gilardini, P. Del Porto, A. Dolei, D. Fioravanti, S. Cochi, E. Manella, V. Colizzi

Research output: Contribution to journalArticlepeer-review

Abstract

The proliferation and development of cytotoxic T cells was investigated in human peripheral blood mononuclear cell (PBMC) cultures stimulated with an antigenic extract from Candida albicans (MPPS), or with the purified protein derivative from Mycobacterium tuberculosis (PPD), or with human recombinant interleukin 2 (rIL-2). Microbial antigen- and rIL-2-induced cytotoxic T cells were able to lyse both natural killer (NK) sensitive and resistant targets. No correlation was observed between the development of T cell cytotoxicity and interferon (IFN) production in vitro. The addition of anti-class II monoclonal antibodies at the beginning of MPPS/PPD-stimulated cultures inhibited the cell proliferation, IFN production and T cell cytotoxicity, while all these cellular activities were not inhibited by anti-class II antibodies in rIL-2-stimulated cultures. Finally, antibodies to class I determinants inhibit T cell cytotoxicity, suggesting a role of such determinants in the development of the non-adaptive immunity to microbial infections.

Original languageEnglish
Pages (from-to)316-329
Number of pages14
JournalFolia Biologica
Volume34
Issue number5
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

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