Induction by carcinogens and chemoprevention by N-acetylcysteine of adducts to mitochondrial DNA in rat organs

Roumen Balansky, Alberto Izzotti, Leonardo Scatolini, Francesco D'Agostini, Silvio De Flora

Research output: Contribution to journalArticlepeer-review

Abstract

Damage to mitochondrial DNA (mtDNA) has been postulated to be associated with aging, cancer, and other chronic degenerative diseases which are the predominant causes of death in the population. We used molecular dosimetry techniques, i.e., 32P postlabeling and synchronous fluorescence spectrophotometry, in order to evaluate the formation of adducts to both mtDNA and nuclear DNA (nDNA) in different organs of rats exposed to genotoxic carcinogens. Adducts to mtDNA were detected following administration of benzo(a)pyrene i.p. or 2-acetylaminofluorene by garage as well as following exposure of animals to cigarette smoke. mtDNA adduct levels were consistently higher than those to nDNA in the same cells, and qualitative differences were also pointed out in the case of the aromatic amine. The oral administration of the thiol N-acetylcysteine, one of the most promising cancer chemopreventive agents endowed with nucleophilic and antioxidant properties, produced a significant decrease of mtDNA adducts in the liver of 2- acetylaminofluorene-treated rats and, sharply, in the lung and liver of rats exposed to cigarette smoke.

Original languageEnglish
Pages (from-to)1642-1647
Number of pages6
JournalCancer Research
Volume56
Issue number7
Publication statusPublished - Apr 1 1996

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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