Induction by transforming growth factor-β1 of the interleukin-1 receptor antagonist and of its intracellular form in human polymorphonuclear cells

Marta Muzio, Marina Sironi, Nadia Polentarutti, Alberto Mantovani, Francesco Colotta

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to examine the expression of interleukin-1 receptor antagonist (IL-1ra) in human polymorphonuclear cells (PMN) treated with transforming growth factor-β1 (TGFβ1). TGFβ1 induced IL-1ra transcripts in human circulating PMN and the induction was not blocked by protein synthesis inhibitors. Actinomycin D blocked induction by TGFβ1 of IL-1ra transcripts, suggesting the involvement of gene transcription. The half life of IL-1ra transcripts was prolonged by TGFβ1. By reverse transcriptase-polymerase chain reaction, TGFβ1 was found to augment the transcripts coding for both the intracellular (keratinocyte type) and the secreted form of IL-1ra. TGFβ1 induced the production of IL-1ra in PMN. Induction of IL-1ra by TGFβ1 in PMN may represent a further mechanism by which this molecule can counteract the potent pro-inflammatory properties of IL-1.

Original languageEnglish
Pages (from-to)3194-3198
Number of pages5
JournalEuropean Journal of Immunology
Volume24
Issue number12
DOIs
Publication statusPublished - Dec 1994

Keywords

  • Interleukin-1 receptor antagonist
  • Polymorphonuclear cells
  • Transforming growth factor-β1

ASJC Scopus subject areas

  • Immunology

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