Abstract
BALB/c 3T3 cells transformed by 1,1,2,2-tetrachloroethane, the most toxic chloroethane, acquired a fully malignant phenotype. They were capable of growing in soft agar, were tumorigenic when injected in athymic mice and produced pulmonary nodules after i.v. injection. Untreated BALB/c 3T3 cells were weakly tumorigenic (3/9 animals developed tumors after a longer time) and induced rare pulmonary nodules in a low percentage of animals. Transformed cells were also invasive in the chemoinvasion assay. Moreover, they grew in a gel of reconstituted basement membrane (matrigel), penetrating the gel and assuming a branching morphology that has been associated to the malignant phenotype. By contrast, spontaneous transformants isolated from solvent controls were poorly invasive in these assays. These results show that 1,1,2,2-tetrachloroethane could play a role in the late steps of multistage carcinogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 937-945 |
| Number of pages | 9 |
| Journal | International Journal of Oncology |
| Volume | 2 |
| Issue number | 6 |
| Publication status | Published - 1993 |
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Keywords
- BALB/c 3T3 cells
- chloroethane
- malignant transformation
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Induction of a malignant phenotype in BALB/c 3T3 cells by 1,1,2,2-tetrachloroethane. / Colacci, A.; Albini, A.; Melchiori, A.; Nanni, P.; Nicoletti, G.; Noonan, D.; Parodi, S.; Grilli, S.
In: International Journal of Oncology, Vol. 2, No. 6, 1993, p. 937-945.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of a malignant phenotype in BALB/c 3T3 cells by 1,1,2,2-tetrachloroethane
AU - Colacci, A.
AU - Albini, A.
AU - Melchiori, A.
AU - Nanni, P.
AU - Nicoletti, G.
AU - Noonan, D.
AU - Parodi, S.
AU - Grilli, S.
PY - 1993
Y1 - 1993
N2 - BALB/c 3T3 cells transformed by 1,1,2,2-tetrachloroethane, the most toxic chloroethane, acquired a fully malignant phenotype. They were capable of growing in soft agar, were tumorigenic when injected in athymic mice and produced pulmonary nodules after i.v. injection. Untreated BALB/c 3T3 cells were weakly tumorigenic (3/9 animals developed tumors after a longer time) and induced rare pulmonary nodules in a low percentage of animals. Transformed cells were also invasive in the chemoinvasion assay. Moreover, they grew in a gel of reconstituted basement membrane (matrigel), penetrating the gel and assuming a branching morphology that has been associated to the malignant phenotype. By contrast, spontaneous transformants isolated from solvent controls were poorly invasive in these assays. These results show that 1,1,2,2-tetrachloroethane could play a role in the late steps of multistage carcinogenesis.
AB - BALB/c 3T3 cells transformed by 1,1,2,2-tetrachloroethane, the most toxic chloroethane, acquired a fully malignant phenotype. They were capable of growing in soft agar, were tumorigenic when injected in athymic mice and produced pulmonary nodules after i.v. injection. Untreated BALB/c 3T3 cells were weakly tumorigenic (3/9 animals developed tumors after a longer time) and induced rare pulmonary nodules in a low percentage of animals. Transformed cells were also invasive in the chemoinvasion assay. Moreover, they grew in a gel of reconstituted basement membrane (matrigel), penetrating the gel and assuming a branching morphology that has been associated to the malignant phenotype. By contrast, spontaneous transformants isolated from solvent controls were poorly invasive in these assays. These results show that 1,1,2,2-tetrachloroethane could play a role in the late steps of multistage carcinogenesis.
KW - BALB/c 3T3 cells
KW - chloroethane
KW - malignant transformation
UR - http://www.scopus.com/inward/record.url?scp=0027158226&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027158226&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0027158226
VL - 2
SP - 937
EP - 945
JO - International Journal of Oncology
JF - International Journal of Oncology
SN - 1019-6439
IS - 6
ER -