TY - JOUR
T1 - Induction of a malignant phenotype in BALB/c 3T3 cells by 1,1,2,2-tetrachloroethane
AU - Colacci, A.
AU - Albini, A.
AU - Melchiori, A.
AU - Nanni, P.
AU - Nicoletti, G.
AU - Noonan, D.
AU - Parodi, S.
AU - Grilli, S.
PY - 1993
Y1 - 1993
N2 - BALB/c 3T3 cells transformed by 1,1,2,2-tetrachloroethane, the most toxic chloroethane, acquired a fully malignant phenotype. They were capable of growing in soft agar, were tumorigenic when injected in athymic mice and produced pulmonary nodules after i.v. injection. Untreated BALB/c 3T3 cells were weakly tumorigenic (3/9 animals developed tumors after a longer time) and induced rare pulmonary nodules in a low percentage of animals. Transformed cells were also invasive in the chemoinvasion assay. Moreover, they grew in a gel of reconstituted basement membrane (matrigel), penetrating the gel and assuming a branching morphology that has been associated to the malignant phenotype. By contrast, spontaneous transformants isolated from solvent controls were poorly invasive in these assays. These results show that 1,1,2,2-tetrachloroethane could play a role in the late steps of multistage carcinogenesis.
AB - BALB/c 3T3 cells transformed by 1,1,2,2-tetrachloroethane, the most toxic chloroethane, acquired a fully malignant phenotype. They were capable of growing in soft agar, were tumorigenic when injected in athymic mice and produced pulmonary nodules after i.v. injection. Untreated BALB/c 3T3 cells were weakly tumorigenic (3/9 animals developed tumors after a longer time) and induced rare pulmonary nodules in a low percentage of animals. Transformed cells were also invasive in the chemoinvasion assay. Moreover, they grew in a gel of reconstituted basement membrane (matrigel), penetrating the gel and assuming a branching morphology that has been associated to the malignant phenotype. By contrast, spontaneous transformants isolated from solvent controls were poorly invasive in these assays. These results show that 1,1,2,2-tetrachloroethane could play a role in the late steps of multistage carcinogenesis.
KW - BALB/c 3T3 cells
KW - chloroethane
KW - malignant transformation
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M3 - Article
AN - SCOPUS:0027158226
VL - 2
SP - 937
EP - 945
JO - International Journal of Oncology
JF - International Journal of Oncology
SN - 1019-6439
IS - 6
ER -