Induction of a malignant phenotype in BALB/c 3T3 cells by 1,1,2,2-tetrachloroethane

A. Colacci, A. Albini, A. Melchiori, P. Nanni, G. Nicoletti, D. Noonan, S. Parodi, S. Grilli

Research output: Contribution to journalArticle

Abstract

BALB/c 3T3 cells transformed by 1,1,2,2-tetrachloroethane, the most toxic chloroethane, acquired a fully malignant phenotype. They were capable of growing in soft agar, were tumorigenic when injected in athymic mice and produced pulmonary nodules after i.v. injection. Untreated BALB/c 3T3 cells were weakly tumorigenic (3/9 animals developed tumors after a longer time) and induced rare pulmonary nodules in a low percentage of animals. Transformed cells were also invasive in the chemoinvasion assay. Moreover, they grew in a gel of reconstituted basement membrane (matrigel), penetrating the gel and assuming a branching morphology that has been associated to the malignant phenotype. By contrast, spontaneous transformants isolated from solvent controls were poorly invasive in these assays. These results show that 1,1,2,2-tetrachloroethane could play a role in the late steps of multistage carcinogenesis.

Original languageEnglish
Pages (from-to)937-945
Number of pages9
JournalInternational Journal of Oncology
Volume2
Issue number6
Publication statusPublished - 1993

Fingerprint

BALB 3T3 Cells
Ethyl Chloride
Gels
Phenotype
Lung
Poisons
Basement Membrane
Nude Mice
Agar
Carcinogenesis
Injections
Neoplasms
1,1,2,2-tetrachloroethane

Keywords

  • BALB/c 3T3 cells
  • chloroethane
  • malignant transformation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Induction of a malignant phenotype in BALB/c 3T3 cells by 1,1,2,2-tetrachloroethane. / Colacci, A.; Albini, A.; Melchiori, A.; Nanni, P.; Nicoletti, G.; Noonan, D.; Parodi, S.; Grilli, S.

In: International Journal of Oncology, Vol. 2, No. 6, 1993, p. 937-945.

Research output: Contribution to journalArticle

Colacci, A. ; Albini, A. ; Melchiori, A. ; Nanni, P. ; Nicoletti, G. ; Noonan, D. ; Parodi, S. ; Grilli, S. / Induction of a malignant phenotype in BALB/c 3T3 cells by 1,1,2,2-tetrachloroethane. In: International Journal of Oncology. 1993 ; Vol. 2, No. 6. pp. 937-945.
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AU - Nanni, P.

AU - Nicoletti, G.

AU - Noonan, D.

AU - Parodi, S.

AU - Grilli, S.

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