Induction of alpha interferon by membrane interaction between viral surface and peripheral blood mononuclear cells

M. R. Capobianchi, J. Facchini, P. Di Marco, G. Antonelli, F. Dianzani

Research output: Contribution to journalArticlepeer-review

Abstract

Cells infected with viruses and fixed when viral antigens appeared at the cell membrane induced much higher alpha interferon (IFN-α) levels in human peripheral blood mononuclear cells (PBMC) than free virions. Relatively few inducer cells were sufficient for triggering IFN production. Optimal IFN yields depended on inducer/producer cell ratio. The response was peculiar to PBMC as it was not found in other cells in which IFN can normally be induced by free virions. IFN inducing activity was also exerted by live virus-infected PBMC, showing that this type of induction may have physiological importance. These findings confirm that viral induction of IFN-α is activated by some interaction between viral components presented at the cell surface and PBMC membrane. Thus induction of IFN by circulating cells infected by viruses and presenting viral antigens at the surface may be an efficient host defense mechanism. Since IFN yields close to 106 international units per millimeter are obtained, this system has potential for large scale production of native IFN-α.

Original languageEnglish
Pages (from-to)551-556
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume178
Issue number4
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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