Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites

Valentina Pacciani, Silvia Gregori, Loredana Chini, Stefania Corrente, Marco Chianca, Viviana Moschese, Paolo Rossi, Maria Grazia Roncarolo, Federica Angelini

Research output: Contribution to journalArticle

Abstract

Background: Dendritic cells (DCs) regulate the immune response to allergens in the lung; they induce either effector or regulatory T cells, which promote or suppress, respectively, the development of allergy. IL-10 is a potent immunosuppressive cytokine that induces type 1 regulatory (Tr1) T cells. Objective: To generate allergen-specific Tr1 cells in vitro from children with allergy. Methods: Monocyte-derived DCs from children with allergy to house dust mites (HDM) were generated by incubating the cells with IL-10 and pulsing them with Der p 2, a major HDM allergen, or by pulsing them with Der p 2 and incubating them with IL-10 during their last 2 days of differentiation. Results: Der p 2-specific T-cell proliferation and TH2 cytokine production were significantly reduced when T cells from patients with allergy to HDM were activated with autologous Der p 2-pulsed DCs that had been differentiated or incubated with IL-10. T-cell lines generated with Der p 2-pulsed DCs that were differentiated with IL-10 were hyporesponsive to reactivation with Der p 2 and able to suppress Der p 2-specific TH2 effector cells. Conclusion: Dendritic cells differentiated in the presence of IL-10 and pulsed with allergen gave rise to a population of tolerogenic DCs that induced allergen-specific Tr1 cells. This finding represents an important step forward to the prospective clinical application of tolerogenic DCs to modulate allergen-specific T-cell responses.

Original languageEnglish
Pages (from-to)727-736
Number of pages10
JournalJournal of Allergy and Clinical Immunology
Volume125
Issue number3
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Pyroglyphidae
Allergens
Dendritic Cells
Hypersensitivity
Interleukin-10
Regulatory T-Lymphocytes
T-Lymphocytes
Cytokines
Dermatophagoides Antigens
Immunosuppressive Agents
Dermatophagoides pteronyssinus antigen p 2
Monocytes
Cell Proliferation
Cell Line
Lung
Population

Keywords

  • Allergy
  • dendritic cells
  • Der p 2
  • house dust mites
  • IL-10
  • T2 cells
  • Tr1 cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites. / Pacciani, Valentina; Gregori, Silvia; Chini, Loredana; Corrente, Stefania; Chianca, Marco; Moschese, Viviana; Rossi, Paolo; Roncarolo, Maria Grazia; Angelini, Federica.

In: Journal of Allergy and Clinical Immunology, Vol. 125, No. 3, 03.2010, p. 727-736.

Research output: Contribution to journalArticle

Pacciani, Valentina ; Gregori, Silvia ; Chini, Loredana ; Corrente, Stefania ; Chianca, Marco ; Moschese, Viviana ; Rossi, Paolo ; Roncarolo, Maria Grazia ; Angelini, Federica. / Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites. In: Journal of Allergy and Clinical Immunology. 2010 ; Vol. 125, No. 3. pp. 727-736.
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AU - Corrente, Stefania

AU - Chianca, Marco

AU - Moschese, Viviana

AU - Rossi, Paolo

AU - Roncarolo, Maria Grazia

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