Abstract
Background: Dendritic cells (DCs) regulate the immune response to allergens in the lung; they induce either effector or regulatory T cells, which promote or suppress, respectively, the development of allergy. IL-10 is a potent immunosuppressive cytokine that induces type 1 regulatory (Tr1) T cells. Objective: To generate allergen-specific Tr1 cells in vitro from children with allergy. Methods: Monocyte-derived DCs from children with allergy to house dust mites (HDM) were generated by incubating the cells with IL-10 and pulsing them with Der p 2, a major HDM allergen, or by pulsing them with Der p 2 and incubating them with IL-10 during their last 2 days of differentiation. Results: Der p 2-specific T-cell proliferation and TH2 cytokine production were significantly reduced when T cells from patients with allergy to HDM were activated with autologous Der p 2-pulsed DCs that had been differentiated or incubated with IL-10. T-cell lines generated with Der p 2-pulsed DCs that were differentiated with IL-10 were hyporesponsive to reactivation with Der p 2 and able to suppress Der p 2-specific TH2 effector cells. Conclusion: Dendritic cells differentiated in the presence of IL-10 and pulsed with allergen gave rise to a population of tolerogenic DCs that induced allergen-specific Tr1 cells. This finding represents an important step forward to the prospective clinical application of tolerogenic DCs to modulate allergen-specific T-cell responses.
| Original language | English |
|---|---|
| Pages (from-to) | 727-736 |
| Number of pages | 10 |
| Journal | Journal of Allergy and Clinical Immunology |
| Volume | 125 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2010 |
Fingerprint
Keywords
- Allergy
- dendritic cells
- Der p 2
- house dust mites
- IL-10
- T2 cells
- Tr1 cells
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites. / Pacciani, Valentina; Gregori, Silvia; Chini, Loredana; Corrente, Stefania; Chianca, Marco; Moschese, Viviana; Rossi, Paolo; Roncarolo, Maria Grazia; Angelini, Federica.
In: Journal of Allergy and Clinical Immunology, Vol. 125, No. 3, 03.2010, p. 727-736.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites
AU - Pacciani, Valentina
AU - Gregori, Silvia
AU - Chini, Loredana
AU - Corrente, Stefania
AU - Chianca, Marco
AU - Moschese, Viviana
AU - Rossi, Paolo
AU - Roncarolo, Maria Grazia
AU - Angelini, Federica
PY - 2010/3
Y1 - 2010/3
N2 - Background: Dendritic cells (DCs) regulate the immune response to allergens in the lung; they induce either effector or regulatory T cells, which promote or suppress, respectively, the development of allergy. IL-10 is a potent immunosuppressive cytokine that induces type 1 regulatory (Tr1) T cells. Objective: To generate allergen-specific Tr1 cells in vitro from children with allergy. Methods: Monocyte-derived DCs from children with allergy to house dust mites (HDM) were generated by incubating the cells with IL-10 and pulsing them with Der p 2, a major HDM allergen, or by pulsing them with Der p 2 and incubating them with IL-10 during their last 2 days of differentiation. Results: Der p 2-specific T-cell proliferation and TH2 cytokine production were significantly reduced when T cells from patients with allergy to HDM were activated with autologous Der p 2-pulsed DCs that had been differentiated or incubated with IL-10. T-cell lines generated with Der p 2-pulsed DCs that were differentiated with IL-10 were hyporesponsive to reactivation with Der p 2 and able to suppress Der p 2-specific TH2 effector cells. Conclusion: Dendritic cells differentiated in the presence of IL-10 and pulsed with allergen gave rise to a population of tolerogenic DCs that induced allergen-specific Tr1 cells. This finding represents an important step forward to the prospective clinical application of tolerogenic DCs to modulate allergen-specific T-cell responses.
AB - Background: Dendritic cells (DCs) regulate the immune response to allergens in the lung; they induce either effector or regulatory T cells, which promote or suppress, respectively, the development of allergy. IL-10 is a potent immunosuppressive cytokine that induces type 1 regulatory (Tr1) T cells. Objective: To generate allergen-specific Tr1 cells in vitro from children with allergy. Methods: Monocyte-derived DCs from children with allergy to house dust mites (HDM) were generated by incubating the cells with IL-10 and pulsing them with Der p 2, a major HDM allergen, or by pulsing them with Der p 2 and incubating them with IL-10 during their last 2 days of differentiation. Results: Der p 2-specific T-cell proliferation and TH2 cytokine production were significantly reduced when T cells from patients with allergy to HDM were activated with autologous Der p 2-pulsed DCs that had been differentiated or incubated with IL-10. T-cell lines generated with Der p 2-pulsed DCs that were differentiated with IL-10 were hyporesponsive to reactivation with Der p 2 and able to suppress Der p 2-specific TH2 effector cells. Conclusion: Dendritic cells differentiated in the presence of IL-10 and pulsed with allergen gave rise to a population of tolerogenic DCs that induced allergen-specific Tr1 cells. This finding represents an important step forward to the prospective clinical application of tolerogenic DCs to modulate allergen-specific T-cell responses.
KW - Allergy
KW - dendritic cells
KW - Der p 2
KW - house dust mites
KW - IL-10
KW - T2 cells
KW - Tr1 cells
UR - http://www.scopus.com/inward/record.url?scp=77649271584&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77649271584&partnerID=8YFLogxK
U2 - 10.1016/j.jaci.2009.12.004
DO - 10.1016/j.jaci.2009.12.004
M3 - Article
C2 - 20153036
AN - SCOPUS:77649271584
VL - 125
SP - 727
EP - 736
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
SN - 0091-6749
IS - 3
ER -