Induction of apoptosis of osteoclasts by targeting transcription factors with decoy molecules

Roberta Piva, Letizia Penolazzi, Margherita Zennaro, Ercolina Bianchini, Eros Magri, Monica Borgatti, Ilaria Lampronti, Elisabetta Lambertini, Elisa Tavanti, Roberto Gambari

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We review the effects of two transcription factor decoy oligonucleotides on apoptosis of human osteoclasts (OCs). The first decoy molecule was designed to inhibit nuclear factor kappa-B (NF-κB) binding to target sequence, the second to increase estrogen receptor (ER) α expression. We found that both decoy molecules are potent inducers of apoptosis of human OCs, associated with increase of caspase 3 activity and decrease of interleukin 6 expression. In addition, we provide evidence indicating that these oligonucleotides are active in vivo in inducing OCs apoptosis. Because OCs are essential for skeletal development and remodeling throughout the life of animal and man, the approach described is of potential clinical importance.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Pages509-516
Number of pages8
Volume1091
DOIs
Publication statusPublished - Dec 2006

Publication series

NameAnnals of the New York Academy of Sciences
Volume1091
ISSN (Print)00778923
ISSN (Electronic)17496632

Keywords

  • Estrogen receptor
  • Gene therapy
  • NF-κB
  • Osteoclast
  • PNA
  • Transcription

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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