Induction of apoptotic cell death by DNA topoisomerase II inhibitors

C. Negri, R. Bernardi, M. Donzelli, A. I. Scovassi

Research output: Contribution to journalArticlepeer-review

Abstract

We have analyzed the interference of antitumoral drugs acting through the inhibition of DNA topoisomerase II on the human HeLa cell metabolism. Different compounds characterized by a diverse mechanism of action have been used, namely m-amsacrine, an intercalative drug, etoposide, which does not intercalate DNA, and suramin, which exerts its effect through an unknown mechanism. In HeLa cells treated with increasing doses of these drugs, we have examined cell viability and DNA synthesis capacity, and we have evaluated topoisomerase II activity. Cellular morphology and DNA integrity have been studied in order to characterize the mechanism of cell death. The results we have obtained clearly indicate that topoisomerase II poisons induce cell death by apoptosis. These observations suggests a role of the inhibition of topoisomerase II activity in the apoptotic program.

Original languageEnglish
Pages (from-to)893-899
Number of pages7
JournalBiochimie
Volume77
Issue number11
DOIs
Publication statusPublished - 1995

Keywords

  • antitumoral drugs
  • apoptosis
  • DNA ladder
  • DNA topoisomerase II

ASJC Scopus subject areas

  • Biochemistry

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