Induction of cross-reactive humoral immune response by immunization with mimotopes of the Hyper Variable Region 1 of the Hepatitis C Virus

R. Roccasecca, A. Folgori, B. B. Ercole, G. Puntoriero, A. Lahm, S. Zucchelli, R. Tafi, M. Pezzanera, G. Galfre, A. Tramontano, M. U. Mondelli, A. Pessi, A. Nicosia, R. Cortese, A. Meola

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Hepatitis C Virus (HCV) is a major cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma, worldwide, and the development of an effective vaccine represents a high priority goal. The Hyper Variable Region 1 (HVR1) of the second Envelope protein (E2) of HCV contains a principal neutralizing determinant, but it is highly variable among different isolates and it is involved in the escape from host immune response. Thus, to be effective, a vaccine should elicit a cross-reacting bumoral response against the majority of viral variants. We show that it is possible to achieve a broadly cross-reactive immune response in rabbits by immunization with mimotopes of the HVR1, selected from a specialized phage library using HCV patients' sera. At least some of the cross-reacting anti-mimotope antibodies, elicited in rabbits, recognize discontinuous epitopes in a manner similar to those induced by the virus in infected patients.

Original languageEnglish
Pages (from-to)289-300
Number of pages12
JournalInternational Reviews of Immunology
Volume20
Issue number2
Publication statusPublished - 2001

Fingerprint

Humoral Immunity
Hepacivirus
Immunization
Vaccines
Rabbits
Chronic Hepatitis
Liver Cirrhosis
Bacteriophages
Epitopes
Anti-Idiotypic Antibodies
Hepatocellular Carcinoma
Viruses
Serum
Proteins

Keywords

  • Cross-reactivity
  • HCV, Hepatitis C virus
  • HVR1, Hyper Variable Region 1
  • Mimotope

ASJC Scopus subject areas

  • Immunology

Cite this

Roccasecca, R., Folgori, A., Ercole, B. B., Puntoriero, G., Lahm, A., Zucchelli, S., ... Meola, A. (2001). Induction of cross-reactive humoral immune response by immunization with mimotopes of the Hyper Variable Region 1 of the Hepatitis C Virus. International Reviews of Immunology, 20(2), 289-300.

Induction of cross-reactive humoral immune response by immunization with mimotopes of the Hyper Variable Region 1 of the Hepatitis C Virus. / Roccasecca, R.; Folgori, A.; Ercole, B. B.; Puntoriero, G.; Lahm, A.; Zucchelli, S.; Tafi, R.; Pezzanera, M.; Galfre, G.; Tramontano, A.; Mondelli, M. U.; Pessi, A.; Nicosia, A.; Cortese, R.; Meola, A.

In: International Reviews of Immunology, Vol. 20, No. 2, 2001, p. 289-300.

Research output: Contribution to journalArticle

Roccasecca, R, Folgori, A, Ercole, BB, Puntoriero, G, Lahm, A, Zucchelli, S, Tafi, R, Pezzanera, M, Galfre, G, Tramontano, A, Mondelli, MU, Pessi, A, Nicosia, A, Cortese, R & Meola, A 2001, 'Induction of cross-reactive humoral immune response by immunization with mimotopes of the Hyper Variable Region 1 of the Hepatitis C Virus', International Reviews of Immunology, vol. 20, no. 2, pp. 289-300.
Roccasecca, R. ; Folgori, A. ; Ercole, B. B. ; Puntoriero, G. ; Lahm, A. ; Zucchelli, S. ; Tafi, R. ; Pezzanera, M. ; Galfre, G. ; Tramontano, A. ; Mondelli, M. U. ; Pessi, A. ; Nicosia, A. ; Cortese, R. ; Meola, A. / Induction of cross-reactive humoral immune response by immunization with mimotopes of the Hyper Variable Region 1 of the Hepatitis C Virus. In: International Reviews of Immunology. 2001 ; Vol. 20, No. 2. pp. 289-300.
@article{91a9260dbef34160b89bde4ad90336f9,
title = "Induction of cross-reactive humoral immune response by immunization with mimotopes of the Hyper Variable Region 1 of the Hepatitis C Virus",
abstract = "Hepatitis C Virus (HCV) is a major cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma, worldwide, and the development of an effective vaccine represents a high priority goal. The Hyper Variable Region 1 (HVR1) of the second Envelope protein (E2) of HCV contains a principal neutralizing determinant, but it is highly variable among different isolates and it is involved in the escape from host immune response. Thus, to be effective, a vaccine should elicit a cross-reacting bumoral response against the majority of viral variants. We show that it is possible to achieve a broadly cross-reactive immune response in rabbits by immunization with mimotopes of the HVR1, selected from a specialized phage library using HCV patients' sera. At least some of the cross-reacting anti-mimotope antibodies, elicited in rabbits, recognize discontinuous epitopes in a manner similar to those induced by the virus in infected patients.",
keywords = "Cross-reactivity, HCV, Hepatitis C virus, HVR1, Hyper Variable Region 1, Mimotope",
author = "R. Roccasecca and A. Folgori and Ercole, {B. B.} and G. Puntoriero and A. Lahm and S. Zucchelli and R. Tafi and M. Pezzanera and G. Galfre and A. Tramontano and Mondelli, {M. U.} and A. Pessi and A. Nicosia and R. Cortese and A. Meola",
year = "2001",
language = "English",
volume = "20",
pages = "289--300",
journal = "International Reviews of Immunology",
issn = "0883-0185",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - Induction of cross-reactive humoral immune response by immunization with mimotopes of the Hyper Variable Region 1 of the Hepatitis C Virus

AU - Roccasecca, R.

AU - Folgori, A.

AU - Ercole, B. B.

AU - Puntoriero, G.

AU - Lahm, A.

AU - Zucchelli, S.

AU - Tafi, R.

AU - Pezzanera, M.

AU - Galfre, G.

AU - Tramontano, A.

AU - Mondelli, M. U.

AU - Pessi, A.

AU - Nicosia, A.

AU - Cortese, R.

AU - Meola, A.

PY - 2001

Y1 - 2001

N2 - Hepatitis C Virus (HCV) is a major cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma, worldwide, and the development of an effective vaccine represents a high priority goal. The Hyper Variable Region 1 (HVR1) of the second Envelope protein (E2) of HCV contains a principal neutralizing determinant, but it is highly variable among different isolates and it is involved in the escape from host immune response. Thus, to be effective, a vaccine should elicit a cross-reacting bumoral response against the majority of viral variants. We show that it is possible to achieve a broadly cross-reactive immune response in rabbits by immunization with mimotopes of the HVR1, selected from a specialized phage library using HCV patients' sera. At least some of the cross-reacting anti-mimotope antibodies, elicited in rabbits, recognize discontinuous epitopes in a manner similar to those induced by the virus in infected patients.

AB - Hepatitis C Virus (HCV) is a major cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma, worldwide, and the development of an effective vaccine represents a high priority goal. The Hyper Variable Region 1 (HVR1) of the second Envelope protein (E2) of HCV contains a principal neutralizing determinant, but it is highly variable among different isolates and it is involved in the escape from host immune response. Thus, to be effective, a vaccine should elicit a cross-reacting bumoral response against the majority of viral variants. We show that it is possible to achieve a broadly cross-reactive immune response in rabbits by immunization with mimotopes of the HVR1, selected from a specialized phage library using HCV patients' sera. At least some of the cross-reacting anti-mimotope antibodies, elicited in rabbits, recognize discontinuous epitopes in a manner similar to those induced by the virus in infected patients.

KW - Cross-reactivity

KW - HCV, Hepatitis C virus

KW - HVR1, Hyper Variable Region 1

KW - Mimotope

UR - http://www.scopus.com/inward/record.url?scp=0035202402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035202402&partnerID=8YFLogxK

M3 - Article

VL - 20

SP - 289

EP - 300

JO - International Reviews of Immunology

JF - International Reviews of Immunology

SN - 0883-0185

IS - 2

ER -