Induction of cross-reactive humoral immune response by immunization with mimotopes of the Hyper Variable Region 1 of the Hepatitis C Virus

R. Roccasecca, A. Folgori, B. B. Ercole, G. Puntoriero, A. Lahm, S. Zucchelli, R. Tafi, M. Pezzanera, G. Galfre, A. Tramontano, M. U. Mondelli, A. Pessi, A. Nicosia, R. Cortese, A. Meola

Research output: Contribution to journalArticle

Abstract

Hepatitis C Virus (HCV) is a major cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma, worldwide, and the development of an effective vaccine represents a high priority goal. The Hyper Variable Region 1 (HVR1) of the second Envelope protein (E2) of HCV contains a principal neutralizing determinant, but it is highly variable among different isolates and it is involved in the escape from host immune response. Thus, to be effective, a vaccine should elicit a cross-reacting bumoral response against the majority of viral variants. We show that it is possible to achieve a broadly cross-reactive immune response in rabbits by immunization with mimotopes of the HVR1, selected from a specialized phage library using HCV patients' sera. At least some of the cross-reacting anti-mimotope antibodies, elicited in rabbits, recognize discontinuous epitopes in a manner similar to those induced by the virus in infected patients.

Original languageEnglish
Pages (from-to)289-300
Number of pages12
JournalInternational Reviews of Immunology
Volume20
Issue number2
Publication statusPublished - 2001

Keywords

  • Cross-reactivity
  • HCV, Hepatitis C virus
  • HVR1, Hyper Variable Region 1
  • Mimotope

ASJC Scopus subject areas

  • Immunology

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