Induction of doxorubicin resistance in heterogeneous human colon tumor cells by N-methylformamide

L. A. Ferrari, S. F. Bliven, M. Wiemann, P. Calabresi, A. S. Glicksman, J. T. Leith

Research output: Contribution to journalArticle

Abstract

Two clonal subpopulations of cells (termed A and D) obtained originally from a heterogeneous human colon adenocarcinoma were studied in vitro with regard to possible modification of responsivity to graded doses of doxorubicin (ADM) after long-term growth in medium containing the differentiating agent N-methylformamide (NMF). Non-NMF-adapted clone A and D cells exhibited biphasic response curves after exposure to graded doses of ADM (0-1.4 μg/ml, 1 hour, 37° C). The inactivation slopes for the initial regions of the survival curves were 0.13 and 0.39 g/ml for clone A and D cells, respectively. NMF-adapted cells, however, exhibited decreased sensitivity to ADM killing as the inactivation slopes for the initial region of the survival curves increased to 0.26 and 0.59 g/ml for clone A and D cells, respectively. The final slopes of the biphasic response curves (doses above about 0.8 μg/ml) were not significantly different between clone A and clone D tumor cells in either the non-NMF- or NMF-treated conditions. These data on ADM responses after differentiation induction by NMF indicate that the combination of differentiation-inducing agents with certain chemotherapeutic agents may produce therapeutically undesirable effects and that preclinical data are necessary prior to implementation of possible combined-modality protocols.

Original languageEnglish
Pages (from-to)1177-1180
Number of pages4
JournalCancer Treatment Reports
Volume70
Issue number10
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Ferrari, L. A., Bliven, S. F., Wiemann, M., Calabresi, P., Glicksman, A. S., & Leith, J. T. (1986). Induction of doxorubicin resistance in heterogeneous human colon tumor cells by N-methylformamide. Cancer Treatment Reports, 70(10), 1177-1180.