Induction of endometriosis in the mouse inhibits spleen leukocyte function

E. Somigliana, P. Viganò, B. Zingrillo, S. Ranieri, P. Filardo, M. Candiani, M. Vignali

Research output: Contribution to journalArticlepeer-review

Abstract

Background. It is still unclear whether the immunologic perturbation observed in women with endometriosis represents an intrinsic defect of the immune system or it is consequent to the presence of endometrium in ectopic sites. The present study was aimed to evaluate the immunoregulatory properties of ectopic endometrial implants in a model of experimental endometriosis in mice. Methods. Endometriosis was induced in n=10 mice by inoculating endometrial fragments obtained from syngenic donor mice into the peritoneal space. Twelve mice were similarly treated but were not inoculated with endometrium and were used as control mice. At an explorative laparotomy performed in all the animals after three weeks, the extent of peritoneal lesions, when present, was evaluated by weight assessment and surface area measurement. In both mice that were inoculated with endometrium and control animals, spleens were removed. The effect of endometriosis induction on concanavalin A-induced spleenocyte proliferation was investigated. Results. A significant inhibition of spleenocyte growth was demonstrated in mice in which endometriosis was induced (36156±3061 cpm) compared to control animals (47172±3210 cpm; p

Original languageEnglish
Pages (from-to)200-205
Number of pages6
JournalActa Obstetricia et Gynecologica Scandinavica
Volume80
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • Endometriosis
  • Mouse model
  • Spleen

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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