Induction of estrogen receptor α expression with decoy oligonucleotide targeted to NFATc1 binding sites in osteoblasts

Letizia Penolazzi, Margherita Zennaro, Elisabetta Lambertini, Elisa Tavanti, Elena Torreggiani, Roberto Gambari, Roberta Piva

Research output: Contribution to journalArticle

Abstract

The nuclear factor of activated T cell cytoplasmic 1 (NFATc1) is a member of the NFAT family and is strictly implicated in the growth and development of bone. Most studies have focused on the effects of NFATc1 activation on osteoclastogenesis. On the contrary, the specific roles of NFAT in osteoblast differentiation are not well understood and, in some instances, reports of its role are contradictory. In the present study, we demonstrated that NFATc1 was involved in the transcriptional regulation of human estrogen receptor α (ERα) gene in SaOS-2 osteoblastic like cells. NFATc1 was specifically recruited "in vivo" at C and F distal promoters of ERα gene. In addition, it is here identified as the negative transcription factor removed by the RA4-3′ decoy oligonucleotide able to induce ERα expression in osteoblasts. Ca2+/calcineurin-NFAT-mediated signaling pathways and ERα-dependent signals are involved in diverse cellular reactions by regulating gene expression under both physiological and pathological conditions. Therefore, our data might be useful for proper manipulation of NFATc1- and ERα- mediated cellular reactions in different bone disorders, such as osteoporosis.

Original languageEnglish
Pages (from-to)1457-1462
Number of pages6
JournalMolecular Pharmacology
Volume71
Issue number6
DOIs
Publication statusPublished - Jun 2007

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ASJC Scopus subject areas

  • Pharmacology

Cite this

Penolazzi, L., Zennaro, M., Lambertini, E., Tavanti, E., Torreggiani, E., Gambari, R., & Piva, R. (2007). Induction of estrogen receptor α expression with decoy oligonucleotide targeted to NFATc1 binding sites in osteoblasts. Molecular Pharmacology, 71(6), 1457-1462. https://doi.org/10.1124/mol.107.034561