Induction of Expandable Tissue-Specific Stem/Progenitor Cells through Transient Expression of YAP/TAZ

Tito Panciera; Luca Azzolin; Atsushi Fujimura; Daniele Di Biagio; Chiara Frasson; Silvia Bresolin; Sandra Soligo; Giuseppe Basso; Silvio Bicciato; Antonio Rosato; Michelangelo Cordenonsi; Stefano Piccolo

doi:10.1016/j.stem.2016.08.009

Induction of Expandable Tissue-Specific Stem/Progenitor Cells through Transient Expression of YAP/TAZ

Tito Panciera, Luca Azzolin, Atsushi Fujimura, Daniele Di Biagio, Chiara Frasson, Silvia Bresolin, Sandra Soligo, Giuseppe Basso, Silvio Bicciato, Antonio Rosato, Michelangelo Cordenonsi, Stefano Piccolo

Istituto Oncologico Veneto

Research output: Contribution to journal › Article

Abstract

The ability to induce autologous tissue-specific stem cells in culture could have a variety of applications in regenerative medicine and disease modeling. Here we show that transient expression of exogenous YAP or its closely related paralogue TAZ in primary differentiated mouse cells can induce conversion to a tissue-specific stem/progenitor cell state. Differentiated mammary gland, neuronal, and pancreatic exocrine cells, identified using a combination of cell sorting and lineage tracing approaches, efficiently convert to proliferating cells with properties of stem/progenitor cells of their respective tissues after YAP induction. YAP-induced mammary stem/progenitor cells show molecular and functional properties similar to endogenous MaSCs, including organoid formation and mammary gland reconstitution after transplantation. Because YAP/TAZ function is also important for self-renewal of endogenous stem cells in culture, our findings have implications for understanding the molecular determinants of the somatic stem cell state.

Original language	English
Pages (from-to)	725-737
Number of pages	13
Journal	Cell Stem Cell
Volume	19
Issue number	6
DOIs	https://doi.org/10.1016/j.stem.2016.08.009
Publication status	Published - Dec 1 2016

ASJC Scopus subject areas

Molecular Medicine
Genetics
Cell Biology

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Panciera, T., Azzolin, L., Fujimura, A., Di Biagio, D., Frasson, C., Bresolin, S., Soligo, S., Basso, G., Bicciato, S., Rosato, A., Cordenonsi, M., & Piccolo, S. (2016). Induction of Expandable Tissue-Specific Stem/Progenitor Cells through Transient Expression of YAP/TAZ. Cell Stem Cell, 19(6), 725-737. https://doi.org/10.1016/j.stem.2016.08.009

Induction of Expandable Tissue-Specific Stem/Progenitor Cells through Transient Expression of YAP/TAZ. / Panciera, Tito; Azzolin, Luca; Fujimura, Atsushi; Di Biagio, Daniele; Frasson, Chiara; Bresolin, Silvia; Soligo, Sandra; Basso, Giuseppe; Bicciato, Silvio; Rosato, Antonio; Cordenonsi, Michelangelo; Piccolo, Stefano.

In: Cell Stem Cell, Vol. 19, No. 6, 01.12.2016, p. 725-737.

Research output: Contribution to journal › Article

Panciera, Tito ; Azzolin, Luca ; Fujimura, Atsushi ; Di Biagio, Daniele ; Frasson, Chiara ; Bresolin, Silvia ; Soligo, Sandra ; Basso, Giuseppe ; Bicciato, Silvio ; Rosato, Antonio ; Cordenonsi, Michelangelo ; Piccolo, Stefano. / Induction of Expandable Tissue-Specific Stem/Progenitor Cells through Transient Expression of YAP/TAZ. In: Cell Stem Cell. 2016 ; Vol. 19, No. 6. pp. 725-737.

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