TY - JOUR
T1 - Induction of Expandable Tissue-Specific Stem/Progenitor Cells through Transient Expression of YAP/TAZ
AU - Panciera, Tito
AU - Azzolin, Luca
AU - Fujimura, Atsushi
AU - Di Biagio, Daniele
AU - Frasson, Chiara
AU - Bresolin, Silvia
AU - Soligo, Sandra
AU - Basso, Giuseppe
AU - Bicciato, Silvio
AU - Rosato, Antonio
AU - Cordenonsi, Michelangelo
AU - Piccolo, Stefano
PY - 2016/12/1
Y1 - 2016/12/1
N2 - The ability to induce autologous tissue-specific stem cells in culture could have a variety of applications in regenerative medicine and disease modeling. Here we show that transient expression of exogenous YAP or its closely related paralogue TAZ in primary differentiated mouse cells can induce conversion to a tissue-specific stem/progenitor cell state. Differentiated mammary gland, neuronal, and pancreatic exocrine cells, identified using a combination of cell sorting and lineage tracing approaches, efficiently convert to proliferating cells with properties of stem/progenitor cells of their respective tissues after YAP induction. YAP-induced mammary stem/progenitor cells show molecular and functional properties similar to endogenous MaSCs, including organoid formation and mammary gland reconstitution after transplantation. Because YAP/TAZ function is also important for self-renewal of endogenous stem cells in culture, our findings have implications for understanding the molecular determinants of the somatic stem cell state.
AB - The ability to induce autologous tissue-specific stem cells in culture could have a variety of applications in regenerative medicine and disease modeling. Here we show that transient expression of exogenous YAP or its closely related paralogue TAZ in primary differentiated mouse cells can induce conversion to a tissue-specific stem/progenitor cell state. Differentiated mammary gland, neuronal, and pancreatic exocrine cells, identified using a combination of cell sorting and lineage tracing approaches, efficiently convert to proliferating cells with properties of stem/progenitor cells of their respective tissues after YAP induction. YAP-induced mammary stem/progenitor cells show molecular and functional properties similar to endogenous MaSCs, including organoid formation and mammary gland reconstitution after transplantation. Because YAP/TAZ function is also important for self-renewal of endogenous stem cells in culture, our findings have implications for understanding the molecular determinants of the somatic stem cell state.
UR - http://www.scopus.com/inward/record.url?scp=84994728929&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84994728929&partnerID=8YFLogxK
U2 - 10.1016/j.stem.2016.08.009
DO - 10.1016/j.stem.2016.08.009
M3 - Article
AN - SCOPUS:84994728929
VL - 19
SP - 725
EP - 737
JO - Cell Stem Cell
JF - Cell Stem Cell
SN - 1934-5909
IS - 6
ER -