Induction of experimental SLE in naive mice by immunization with human polyclonal anti-DNA antibody carrying the 16/6 idiotype

A. Tincani, G. Balestrieri, F. Allegri, R. Cattaneo, A. Fornasieri, M. Li, A. Sinico, G. D'Amico

Research output: Contribution to journalArticle

Abstract

Recently, the induction of SLE in naive mice employing monoclonal anti-DNA antibodies (anti-DNA Ab) carrying the pathogenic idiotype 16/6 (16/6 Id) has been reported. In the current study we report on the induction of experimental SLE by polyclonal IgG anti-DNA Ab derived from a patient with active SLE and carrying the 16/6 Id. Two different experiments were conducted in which BALB/c mice were immunized in the foot pads with 1μg/ml or 5μg/ml of anti DNA Ab. The first experiment showed the appearence in the immunized mice of high titre anti-DNA Ab together with antinuclear antibodies, alopecia and proteinuria. In the second experiment we compared, as immunizing agents, 16/6 positive anti-DNA Ab and 16/6 negative anti-tetanus toxoid antibodies (anti-TT Ab) obtained from the serum of the same patient. Our results show that only mice immunized with 16/6 positive antibodies produced anti-DNA Ab, while mice immunized with anti-TT Ab did not show any DNA-binding activity but, surprisingly, developed high titre anti-cardiolipin antibodies.

Original languageEnglish
Pages (from-to)129-134
Number of pages6
JournalClinical and Experimental Rheumatology
Volume11
Issue number2
Publication statusPublished - 1993

Fingerprint

Antinuclear Antibodies
Immunization
Tetanus Toxoid
Antibodies
Cardiolipins
DNA
Alopecia
Proteinuria
Foot
Anti-Idiotypic Antibodies
Immunoglobulin G
Monoclonal Antibodies
Serum

Keywords

  • 16/6 idiotype
  • animal model
  • anti-DNA antibodies
  • experimental SLE
  • idiotype

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Tincani, A., Balestrieri, G., Allegri, F., Cattaneo, R., Fornasieri, A., Li, M., ... D'Amico, G. (1993). Induction of experimental SLE in naive mice by immunization with human polyclonal anti-DNA antibody carrying the 16/6 idiotype. Clinical and Experimental Rheumatology, 11(2), 129-134.

Induction of experimental SLE in naive mice by immunization with human polyclonal anti-DNA antibody carrying the 16/6 idiotype. / Tincani, A.; Balestrieri, G.; Allegri, F.; Cattaneo, R.; Fornasieri, A.; Li, M.; Sinico, A.; D'Amico, G.

In: Clinical and Experimental Rheumatology, Vol. 11, No. 2, 1993, p. 129-134.

Research output: Contribution to journalArticle

Tincani, A, Balestrieri, G, Allegri, F, Cattaneo, R, Fornasieri, A, Li, M, Sinico, A & D'Amico, G 1993, 'Induction of experimental SLE in naive mice by immunization with human polyclonal anti-DNA antibody carrying the 16/6 idiotype', Clinical and Experimental Rheumatology, vol. 11, no. 2, pp. 129-134.
Tincani, A. ; Balestrieri, G. ; Allegri, F. ; Cattaneo, R. ; Fornasieri, A. ; Li, M. ; Sinico, A. ; D'Amico, G. / Induction of experimental SLE in naive mice by immunization with human polyclonal anti-DNA antibody carrying the 16/6 idiotype. In: Clinical and Experimental Rheumatology. 1993 ; Vol. 11, No. 2. pp. 129-134.
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