Abstract
Recently, the induction of SLE in naive mice employing monoclonal anti-DNA antibodies (anti-DNA Ab) carrying the pathogenic idiotype 16/6 (16/6 Id) has been reported. In the current study we report on the induction of experimental SLE by polyclonal IgG anti-DNA Ab derived from a patient with active SLE and carrying the 16/6 Id. Two different experiments were conducted in which BALB/c mice were immunized in the foot pads with 1μg/ml or 5μg/ml of anti DNA Ab. The first experiment showed the appearence in the immunized mice of high titre anti-DNA Ab together with antinuclear antibodies, alopecia and proteinuria. In the second experiment we compared, as immunizing agents, 16/6 positive anti-DNA Ab and 16/6 negative anti-tetanus toxoid antibodies (anti-TT Ab) obtained from the serum of the same patient. Our results show that only mice immunized with 16/6 positive antibodies produced anti-DNA Ab, while mice immunized with anti-TT Ab did not show any DNA-binding activity but, surprisingly, developed high titre anti-cardiolipin antibodies.
| Original language | English |
|---|---|
| Pages (from-to) | 129-134 |
| Number of pages | 6 |
| Journal | Clinical and Experimental Rheumatology |
| Volume | 11 |
| Issue number | 2 |
| Publication status | Published - 1993 |
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Keywords
- 16/6 idiotype
- animal model
- anti-DNA antibodies
- experimental SLE
- idiotype
ASJC Scopus subject areas
- Immunology
- Rheumatology
Cite this
Induction of experimental SLE in naive mice by immunization with human polyclonal anti-DNA antibody carrying the 16/6 idiotype. / Tincani, A.; Balestrieri, G.; Allegri, F.; Cattaneo, R.; Fornasieri, A.; Li, M.; Sinico, A.; D'Amico, G.
In: Clinical and Experimental Rheumatology, Vol. 11, No. 2, 1993, p. 129-134.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of experimental SLE in naive mice by immunization with human polyclonal anti-DNA antibody carrying the 16/6 idiotype
AU - Tincani, A.
AU - Balestrieri, G.
AU - Allegri, F.
AU - Cattaneo, R.
AU - Fornasieri, A.
AU - Li, M.
AU - Sinico, A.
AU - D'Amico, G.
PY - 1993
Y1 - 1993
N2 - Recently, the induction of SLE in naive mice employing monoclonal anti-DNA antibodies (anti-DNA Ab) carrying the pathogenic idiotype 16/6 (16/6 Id) has been reported. In the current study we report on the induction of experimental SLE by polyclonal IgG anti-DNA Ab derived from a patient with active SLE and carrying the 16/6 Id. Two different experiments were conducted in which BALB/c mice were immunized in the foot pads with 1μg/ml or 5μg/ml of anti DNA Ab. The first experiment showed the appearence in the immunized mice of high titre anti-DNA Ab together with antinuclear antibodies, alopecia and proteinuria. In the second experiment we compared, as immunizing agents, 16/6 positive anti-DNA Ab and 16/6 negative anti-tetanus toxoid antibodies (anti-TT Ab) obtained from the serum of the same patient. Our results show that only mice immunized with 16/6 positive antibodies produced anti-DNA Ab, while mice immunized with anti-TT Ab did not show any DNA-binding activity but, surprisingly, developed high titre anti-cardiolipin antibodies.
AB - Recently, the induction of SLE in naive mice employing monoclonal anti-DNA antibodies (anti-DNA Ab) carrying the pathogenic idiotype 16/6 (16/6 Id) has been reported. In the current study we report on the induction of experimental SLE by polyclonal IgG anti-DNA Ab derived from a patient with active SLE and carrying the 16/6 Id. Two different experiments were conducted in which BALB/c mice were immunized in the foot pads with 1μg/ml or 5μg/ml of anti DNA Ab. The first experiment showed the appearence in the immunized mice of high titre anti-DNA Ab together with antinuclear antibodies, alopecia and proteinuria. In the second experiment we compared, as immunizing agents, 16/6 positive anti-DNA Ab and 16/6 negative anti-tetanus toxoid antibodies (anti-TT Ab) obtained from the serum of the same patient. Our results show that only mice immunized with 16/6 positive antibodies produced anti-DNA Ab, while mice immunized with anti-TT Ab did not show any DNA-binding activity but, surprisingly, developed high titre anti-cardiolipin antibodies.
KW - 16/6 idiotype
KW - animal model
KW - anti-DNA antibodies
KW - experimental SLE
KW - idiotype
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UR - http://www.scopus.com/inward/citedby.url?scp=0027190422&partnerID=8YFLogxK
M3 - Article
C2 - 7685255
AN - SCOPUS:0027190422
VL - 11
SP - 129
EP - 134
JO - Clinical and Experimental Rheumatology
JF - Clinical and Experimental Rheumatology
SN - 0392-856X
IS - 2
ER -