Induction of experimental SLE in naive mice by immunization with human polyclonal anti-DNA antibody carrying the 16/6 idiotype

A. Tincani, G. Balestrieri, F. Allegri, R. Cattaneo, A. Fornasieri, M. Li, A. Sinico, G. D'Amico

Research output: Contribution to journalArticlepeer-review

Abstract

Recently, the induction of SLE in naive mice employing monoclonal anti-DNA antibodies (anti-DNA Ab) carrying the pathogenic idiotype 16/6 (16/6 Id) has been reported. In the current study we report on the induction of experimental SLE by polyclonal IgG anti-DNA Ab derived from a patient with active SLE and carrying the 16/6 Id. Two different experiments were conducted in which BALB/c mice were immunized in the foot pads with 1μg/ml or 5μg/ml of anti DNA Ab. The first experiment showed the appearence in the immunized mice of high titre anti-DNA Ab together with antinuclear antibodies, alopecia and proteinuria. In the second experiment we compared, as immunizing agents, 16/6 positive anti-DNA Ab and 16/6 negative anti-tetanus toxoid antibodies (anti-TT Ab) obtained from the serum of the same patient. Our results show that only mice immunized with 16/6 positive antibodies produced anti-DNA Ab, while mice immunized with anti-TT Ab did not show any DNA-binding activity but, surprisingly, developed high titre anti-cardiolipin antibodies.

Original languageEnglish
Pages (from-to)129-134
Number of pages6
JournalClinical and Experimental Rheumatology
Volume11
Issue number2
Publication statusPublished - 1993

Keywords

  • 16/6 idiotype
  • animal model
  • anti-DNA antibodies
  • experimental SLE
  • idiotype

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

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