Abstract
Recently, the induction of SLE in naive mice employing monoclonal anti-DNA antibodies (anti-DNA Ab) carrying the pathogenic idiotype 16/6 (16/6 Id) has been reported. In the current study we report on the induction of experimental SLE by polyclonal IgG anti-DNA Ab derived from a patient with active SLE and carrying the 16/6 Id. Two different experiments were conducted in which BALB/c mice were immunized in the foot pads with 1μg/ml or 5μg/ml of anti DNA Ab. The first experiment showed the appearence in the immunized mice of high titre anti-DNA Ab together with antinuclear antibodies, alopecia and proteinuria. In the second experiment we compared, as immunizing agents, 16/6 positive anti-DNA Ab and 16/6 negative anti-tetanus toxoid antibodies (anti-TT Ab) obtained from the serum of the same patient. Our results show that only mice immunized with 16/6 positive antibodies produced anti-DNA Ab, while mice immunized with anti-TT Ab did not show any DNA-binding activity but, surprisingly, developed high titre anti-cardiolipin antibodies.
Original language | English |
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Pages (from-to) | 129-134 |
Number of pages | 6 |
Journal | Clinical and Experimental Rheumatology |
Volume | 11 |
Issue number | 2 |
Publication status | Published - 1993 |
Keywords
- 16/6 idiotype
- animal model
- anti-DNA antibodies
- experimental SLE
- idiotype
ASJC Scopus subject areas
- Immunology
- Rheumatology