Induction of G2-M phase arrest and apoptosis by α-methylene-γ-butyrolactones in human leukemia cells

Fernando Torres, José Quintana, Javier Cabrera, Juan F. Loro, Francisco León, Jaime Bermejo, Francisco Estévez

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In this study, we investigated the effect of three synthetic α-methylene-γ-butyrolactones (MBL) on viability of 10 human tumor cell lines and found that these lactones were highly cytotoxic against leukemia cells. Studies performed on HL-60 cells indicate that MBL induce G2-M arrest and apoptosis through a caspase-dependent mechanism. Apoptosis was associated to cytochrome c release, cleavage of procaspases-9 and -3, and hydrolysis of PARP. Intracellular reactive oxygen species (ROS) seem to play a key role since high levels of ROS were produced early (

Original languageEnglish
Pages (from-to)139-147
Number of pages9
JournalCancer Letters
Volume269
Issue number1
DOIs
Publication statusPublished - Sep 28 2008

Fingerprint

G2 Phase
Cell Division
Reactive Oxygen Species
Leukemia
Apoptosis
Caspase 9
HL-60 Cells
Lactones
Caspases
Cytochromes c
Tumor Cell Line
Caspase 3
Hydrolysis

Keywords

  • α-Methylene-γ-butyrolactones
  • Apoptosis
  • Caspases
  • DNA fragmentation
  • PARP cleavage
  • Reactive oxygen species

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Induction of G2-M phase arrest and apoptosis by α-methylene-γ-butyrolactones in human leukemia cells. / Torres, Fernando; Quintana, José; Cabrera, Javier; Loro, Juan F.; León, Francisco; Bermejo, Jaime; Estévez, Francisco.

In: Cancer Letters, Vol. 269, No. 1, 28.09.2008, p. 139-147.

Research output: Contribution to journalArticle

Torres, F, Quintana, J, Cabrera, J, Loro, JF, León, F, Bermejo, J & Estévez, F 2008, 'Induction of G2-M phase arrest and apoptosis by α-methylene-γ-butyrolactones in human leukemia cells', Cancer Letters, vol. 269, no. 1, pp. 139-147. https://doi.org/10.1016/j.canlet.2008.04.028
Torres, Fernando ; Quintana, José ; Cabrera, Javier ; Loro, Juan F. ; León, Francisco ; Bermejo, Jaime ; Estévez, Francisco. / Induction of G2-M phase arrest and apoptosis by α-methylene-γ-butyrolactones in human leukemia cells. In: Cancer Letters. 2008 ; Vol. 269, No. 1. pp. 139-147.
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