Induction of graft versus host disease in SCID mice by MRL/lpr cell transfer

M. Fraziano, C. Montesano, I. Sammarco, S. Di cEsare, M. C. Caroleo, M. Mattei, S. Cannata, F. Poccia, A. Bellavia, A. Salerno, V. Colizzi

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We have investigated the graft versus host (GvH) disease induced in immunodeficient SCID (H-2(d)) mice by intravenous ((iv) or intraperitoneal (ip) transfer of either spleen or lymph node cells from autoimmune (MRL/lpr and MRL/++ mice, H-2(k)) and normal (CBA, H-2(k)) mice. Rapid and lethal GvH disease was observed when cells from MRL/lpr or MRL/ ++ were iv transferred into SCID mice, while spleen cells from nonautoimmune CBA donors were partially tolerized into SCID recipients and induced only lower levels of GvH reaction. No GvH reaction (complete tolerance) was observed when CBA lymph node cells were iv transferred into SCID recipients. In contrast, the ip injection of MRL/lpr or CBA spleen cells induces similar levels of GvH. The development of GvH disease in SCID recipients was due to the expansion of alloreactive GD8+ cells displaying significant cytotoxic activity against H-2(d), but not against autologous targets. Also, a significant decrease of CD4/CD8 ratio was observed in the course of GvH caused by the iv transfer of cells from MRL/lpr mice. Altogether, these data support the hypothesis that lymphocytes from the MRL/lpr mice may

Original languageEnglish
Pages (from-to)265-272
Number of pages8
JournalClinical Immunology and Immunopathology
Issue number3
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

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